Immune cell infiltration associated with tumor capsule disruption and tumor budding has been shown to reflect invasiveness, metastasis, and unfavorable prognosis in colorectal cancer. We investigated the influence of tumor budding on prognosis and its association with the immune microenvironment in lung adenocarcinoma.
Tumor slides from resected stage I lung adenocarcinomas were reviewed (n = 524 and n = 514, for training and validation cohorts, respectively) for assessment of tumor budding. CD3+ and FoxP3+ lymphocytes, CD68+ macrophages, IL-7R, and IL-12Rβ2 were analyzed using tissue microarrays constructed from tumor and stroma. Probability of recurrence was calculated using competing risks method.
In the training cohort, risk of recurrence for high-grade tumor budding was higher than it was for low-grade tumor budding (32% vs. 12%, P < 0.001), which was confirmed in the validation cohort (P = 0.005). Tumor budding stratified risk of recurrence for acinar-predominant (22% vs. 9%, P < 0.001), papillary-predominant (22% vs. 13%, P = 0.045), and solid-predominant (39% vs. 19%, P = 0.022) tumors. Tumor budding was associated with higher stromal FoxP3+ lymphocyte infiltration, higher stromal FoxP3/CD3 risk index, higher tumoral and stromal CD68+ macrophage infiltration, and IL-7R overexpression (P < 0.001, all associations). Tumor budding remained independently associated with recurrence on multivariate analysis (HR, 1.61; P = 0.008).
Tumor budding is an independent prognostic factor of stage I lung adenocarcinoma and correlates with protumor immune microenvironment. Our findings advocate investigating tumor-immune cell interactions at invading edge as a biological driver of tumor aggressiveness.