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Poster Walks: Poster Walk 6: Basic Science/Pulmonary hypertension |

P208 Nicotine exposure during gestation and lactation affects cell proliferation and lung volume during lung development in mice

S. Blaskovic; F. Zanetti; Y. Donati; S. Lameille; T.P. Cremona; I. Ruchonnet-Metrailler; J. Schittny; C. Barazzone-Argiroffo
Author and Funding Information

1Department of Pathology and Immunology, University of Geneva, CMU, Genève

2Department of Pediatrics, Children's Hospital, Geneva

3Department of Anatomy, University of Bern, Bern, Switzerland


Copyright 2017, American College of Chest Physicians and Swiss Respiratory Society SGP. All Rights Reserved.


Chest. 2017;151(5_S):A107. doi:10.1016/j.chest.2017.04.113
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Introduction: To ensure adequate gas exchange lung undergoes through different phases of development. Oxygen exchange becomes functional during alveolar phase characterised by septation of existing air sacs and microvascular maturation. Lung development can be affected by several genetic and environmental factors such as cigarettes smoke (CS) .Epidemiological studies showed that exposure to CS during lung development can have an immediate effect, as low birth weight and impaired lung function at birth, but can also manifest later in life making the offspring more susceptible to cardiovascular diseases, obesity and diabetes. The main addictive component of CS is nicotine that can freely cross placenta and accumulate in mother's milk. Since detrimental effects of nicotine are well described, the mechanism of action of long term effect are still vague. Therefore, the aim of this study is to investigate the effect of nicotine during lung development, regarding the processes of cell proliferation and apoptosis during alveolar phase. Since the alveolar phase occurs after birth in mice, we took advantage of this model for our study. We exposed mice to nicotine in drinking water, during gestation and lactation, and collected pups at postnatal day (PND) 2, 8 and 16. The concentration used is 200 mg/L which corresponds to a medium to heavy smoker. Pups' lungs were further processed for gene and protein expression and stereological analysis.

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