SESSION TITLE: Unique Investigations in Interventional Pulmonology
SESSION TYPE: Original Investigation Slide
PRESENTED ON: Tuesday, October 25, 2016 at 02:45 PM - 04:15 PM
PURPOSE: To investigate a novel method of processing specimens obtained in the operating room by endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) which utilizes intraoperative consultation and frozen section (cryosection) to provide a rapid pathologic diagnosis. The frozen section process consists of embedding the specimen in Optimal Cutting Temperature (OCT) compound, freezing to -30C in liquid nitrogen and sectioning the block in a Leica CM1860UV cryostat to a thickness of 5 micrometers. The sections are then stained and mounted on slides for microscopic analysis. This technique allows for histopathologic analysis of intact tissue rather than aggregated cells in a cytologic sample, as in rapid on-site cytologic evaluations (ROSE) that are commonly used for EBUS-TBNA samples.