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Procedures: Unique Investigations in Interventional Pulmonology |

Intraoperative Frozen Section of Endobronchial Ultrasound Transbronchial Needle Aspiration (EBUS-TBNA) Specimens

Bernard Kim, MD; Darling Ruiz Cerrato, MD; Kala Davis-McDonald, MD
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Saint Agnes Hospital, Baltimore, MD


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;150(4_S):1047A. doi:10.1016/j.chest.2016.08.1153
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SESSION TITLE: Unique Investigations in Interventional Pulmonology

SESSION TYPE: Original Investigation Slide

PRESENTED ON: Tuesday, October 25, 2016 at 02:45 PM - 04:15 PM

PURPOSE: To investigate a novel method of processing specimens obtained in the operating room by endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) which utilizes intraoperative consultation and frozen section (cryosection) to provide a rapid pathologic diagnosis. The frozen section process consists of embedding the specimen in Optimal Cutting Temperature (OCT) compound, freezing to -30C in liquid nitrogen and sectioning the block in a Leica CM1860UV cryostat to a thickness of 5 micrometers. The sections are then stained and mounted on slides for microscopic analysis. This technique allows for histopathologic analysis of intact tissue rather than aggregated cells in a cytologic sample, as in rapid on-site cytologic evaluations (ROSE) that are commonly used for EBUS-TBNA samples.

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