METHODS: ViziShot FLEX 19G needles (Olympus Respiratory America, Redmond, WA) and ViziShot 21G and 22G needles (Olympus, Tokyo, Japan) were evaluated under controlled conditions. Fresh pieces of bovine heart and liver were used as substrates. Heart tissue was chosen as a surrogate for hypertrophic, dense lymph node and liver served as surrogate for normal lymph node. Five devices of each kind were tested. Each needle was penetrated by hand 2cm into the tissue target at an angle resembling EBUS-TBNA. Ten agitations using 20cc of vacuum were performed consistently to collect samples. Each sample was ejected using air onto slides. The tissue core was then arranged using a fine needle to remove any overlapping and stacking. The samples were photographed using a high power microscope. The sample images were analyzed using image analysis software (ImageJ, NIH, Bethesda, MD) to calculate the tissue core area. To obtain sample volume, the sample depth was extrapolated from the needles’ inner diameter and then the tissue core area of each sample was multiplied by the sample depth. The sample volume collected by each needle was statistically compared using an unpaired t-test.