SESSION TITLE: Novel Methods for Lung Cancer Diagnosis
SESSION TYPE: Original Investigation Slide
PRESENTED ON: Tuesday, October 25, 2016 at 08:45 AM - 09:45 AM
PURPOSE: The aim of this study is to identify a highly sensitive molecular biomarker during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) staging.
METHODS: Candidate miRNAs were selected from previous results of miRNA microarray analysis (Ann Thorac Surg. 2012). Five miRNAs (miR 200a, miR 200b, miR 200c, miR 141, let-7e) were selected as biomarkers for the detection of nodal metastasis by PCR based on miRNA expression analysis. To determine the cut-off expression level to differentiate malignant from benign lymph nodes, we first analyzed 22 malignant and 47 benign formalin fixed paraffin-embedded (FFPE) surgically resected lymph nodes. We then applied the cut-off expression level to the snap frozen EBUS-TBNA samples. Archived FFPE EBUS-TBNA rebiopsied samples post induction chemo-radiotherapy were also analyzed. The final diagnoses of EBUS-TBNA were made by pathology or clinical follow-up over six months.