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Lung Cancer: Novel Methods for Lung Cancer Diagnosis |

Molecular Nodal Staging Using Mirna Expression in Lung Cancer Patients by EBUS-TBNA

Terunaga Inage, MD; Takahiro Nakajima, PhD; Hironobu Wada, PhD; Taiki Fujiwara, PhD; Hidemi Suzuki, PhD; Takekazu Iwata, PhD; Shigetoshi Yoshida, PhD; Kazuhiro Yasufuku, PhD; Ichiro Yoshino, PhD
Author and Funding Information

Chiba University Graduate School of Medicine, Chiba, Japan


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;150(4_S):735A. doi:10.1016/j.chest.2016.08.830
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SESSION TITLE: Novel Methods for Lung Cancer Diagnosis

SESSION TYPE: Original Investigation Slide

PRESENTED ON: Tuesday, October 25, 2016 at 08:45 AM - 09:45 AM

PURPOSE: The aim of this study is to identify a highly sensitive molecular biomarker during endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) staging.

METHODS: Candidate miRNAs were selected from previous results of miRNA microarray analysis (Ann Thorac Surg. 2012). Five miRNAs (miR 200a, miR 200b, miR 200c, miR 141, let-7e) were selected as biomarkers for the detection of nodal metastasis by PCR based on miRNA expression analysis. To determine the cut-off expression level to differentiate malignant from benign lymph nodes, we first analyzed 22 malignant and 47 benign formalin fixed paraffin-embedded (FFPE) surgically resected lymph nodes. We then applied the cut-off expression level to the snap frozen EBUS-TBNA samples. Archived FFPE EBUS-TBNA rebiopsied samples post induction chemo-radiotherapy were also analyzed. The final diagnoses of EBUS-TBNA were made by pathology or clinical follow-up over six months.

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