SESSION TITLE: Biotechnology
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM
PURPOSE: To examine the effects of simulated microgravity on metastatic ability of the different cell lines of human lung adenocarcinoma and squamous cell carcinoma.
METHODS: Human lung cancer cell lines of adenocarcinoma(A549) and squamous cell carcinoma(H1703) were cultured and maintained in a humidified incubator at 37 C under a 5% CO2 atmosphere. A clinostat system(3D clinostat ver.2) was used for creating simulated microgravity environment and cells were plated into a membrane cell culture dish. The cells adhered to the flask were fixed carefully at the rotating panel of clinostat system, which was placed in the humidified incubator at 37 C under a 5% CO2 atmosphere. The clinostat was continuously rotated at 5 rpm for 36 hours. The control group was cultured in the same way as the experimental group with the exception of clinorotation. In order to analyze the effect of microgravity on proliferation and migration abilities of lung cancer cells, cell proliferation assay, and wound healing scratch assay were performed. And also, to analyze the microgravital effect on the expression levels of biomarkers related to cell migration and invasion, real-time PCR assay and western blot assay of MMP2, MMP9, TIMP-1, TIMP-2 were performed.