0
Biotechnology: Biotechnology |

Effects of Simulated Microgravital Environment on Lung Cancer Cells

Jae Ho Chung; Eunjue Yi; Chi Bum Ahn; Sungho Lee; Kwang Taik Kim
Author and Funding Information

Korea University Medical College, Korea University Anam Hospital, Seoul, Korea (the Republic of)


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;150(4_S):26A. doi:10.1016/j.chest.2016.08.032
Text Size: A A A
Published online

Extract

SESSION TITLE: Biotechnology

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM

PURPOSE: To examine the effects of simulated microgravity on metastatic ability of the different cell lines of human lung adenocarcinoma and squamous cell carcinoma.

METHODS: Human lung cancer cell lines of adenocarcinoma(A549) and squamous cell carcinoma(H1703) were cultured and maintained in a humidified incubator at 37 C under a 5% CO2 atmosphere. A clinostat system(3D clinostat ver.2) was used for creating simulated microgravity environment and cells were plated into a membrane cell culture dish. The cells adhered to the flask were fixed carefully at the rotating panel of clinostat system, which was placed in the humidified incubator at 37 C under a 5% CO2 atmosphere. The clinostat was continuously rotated at 5 rpm for 36 hours. The control group was cultured in the same way as the experimental group with the exception of clinorotation. In order to analyze the effect of microgravity on proliferation and migration abilities of lung cancer cells, cell proliferation assay, and wound healing scratch assay were performed. And also, to analyze the microgravital effect on the expression levels of biomarkers related to cell migration and invasion, real-time PCR assay and western blot assay of MMP2, MMP9, TIMP-1, TIMP-2 were performed.

First Page Preview

View Large
First page PDF preview

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Topics

lung cancer

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543