Biotechnology: Biotechnology |

Biomechanical Properties of the Ex Vivo Porcine Trachea: A Benchmark for 3-Dimensional Bioprinted Airway Replacements FREE TO VIEW

Rachel Kaye, MD; Todd Goldstein, MS; Daniel Grande, PhD; Lee Smith, MD; David Zeltsman, MD
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Montefiore Medical Center, Bronx, NY

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;150(4_S):23A. doi:10.1016/j.chest.2016.08.029
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SESSION TITLE: Biotechnology

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM

PURPOSE: To define the rotation, axial stretch capacity, and positive intraluminal pressure capabilities for ex vivo porcine tracheas. These actions are clinically relevant to the human airway and have not been previously studied in tracheal segments.

METHODS: Ex vivo porcine tracheas were harvested from the cricoid to the carina (average length was 11cm). These full-length tracheal specimens were sectioned into two equal segments with each section approximately 5.5cm long (containing at least 5 cartilaginous rings). The tracheal segments were tested for their maximal positive intraluminal pressure, axial lengthening, and degree of rotation. To measure intraluminal pressure, each end of an ex vivo tracheal segment was sealed with a custom designed and 3-dimensional (3D) printed cap and a pressure transducer was placed through one end to measure the intratracheal pressure. Tracheal length was evaluated by stretching the tracheal segments on their axis between two clamps and using ImageJ software (National Institutes of Health, Bethesda, MD) for measurements. Similarly, tracheal rotation was evaluated by rotating the tracheas between two clamps.

RESULTS: Six tracheal segments were tested for axial lengthening and the post-stretch length percentage was on average 148.92% (range 136.81-163.48%, 95% CI 153-143%). The amount of percentage stretch per cartilaginous ring was on average 23.88% (range 15.74-31.02%, 95% CI 26.68-21.08). Four tracheal segments were tested for maximal positive intraluminal pressure, which was in excess of the limits of our pressure transducer (over 400mmHg). Tracheal segments were tested for degree of rotation and they were found to easily transform to 90 degrees in the anterior-posterior and lateral directions and to 180 degrees when rotated around their axis.

CONCLUSIONS: The maximal positive intraluminal pressure far exceeds that known in the normal respiratory cycle in humans. Likewise the 90 degree bending and 180 degree rotation also exceeds what is expected in vivo. A maximal increase of 148.92% of tracheal axial length is critical to define in order to not put undue tension on anastomosis sites when planning suitable tracheal reconstruction methods.

CLINICAL IMPLICATIONS: This study defines the biomechanical properties of the ex vivo porcine trachea. This is necessary in order to compare the properties of new and innovative 3D bioprinted airway replacement grafts with native tracheal properties.

DISCLOSURE: The following authors have nothing to disclose: Rachel Kaye, Todd Goldstein, Daniel Grande, Lee Smith, David Zeltsman

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