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Original Research |

Variation of ciliary beat pattern in 3 different beating planes in healthy subjects

Celine Kempeneers, MD; Claire Seaton, BM BCh; Mark A. Chilvers, MB ChB MD
Author and Funding Information

Conflict of interests: No potential conflicts of interest with any companies/organizations whose products or services may be discussed in this article exist for the specified authors.

Funding information: This study was supported by the BCCH Telethon Grant. Dr Celine Kempeneers has received the following grants: “Citadelle Recherche et Formation” Grant from CHR, Liege, Belgium, Horlait-Daspens Foundation Grant, Belgium

Notation of prior abstract publication/presentation:

- Kempeneers C, Seaton C, Chilvers MA - Ciliary Videomicroscopy: Defining a Standard Methodology. 2014 International Conference of the American Thoracic Society, San Diego, CA, USA, May 16th-21st, 2014

Kempeneers C, Seaton C, Chilvers MA - Primary Ciliary Dyskinesia Diagnosis: variation of the Ciliary Beat Pattern in Healthy Subjects - 44th Annual Congress of the Belgian Society of Pediatrics (BVK-SBP), Brussels, Belgium, March 10th-11th, 2016

Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children’s Hospital, Vancouver, BC, Canada

Corresponding author information : M. Chilvers, Division of Respirology, BC Children’s Hospital, 4480 Oak Street, Vancouver, BC, V6H 3V4, Canada


Copyright 2016, . All Rights Reserved.


Chest. 2016. doi:10.1016/j.chest.2016.09.015
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Abstract

Background  Digital high speed videomicroscopy(DHSV) allows analysis of ciliary beat frequency(CBF) and pattern(CBP) of respiratory cilia in 3 planes. Normal reference data use a sideways edge to evaluate ciliary dyskinesia, and calculate CBF using the time for a cilium to complete 10 beat cycles. Variability in CBF within respiratory epithelium has been described, but data concerning variation of CBP is limited in healthy epithelium. This study aims to document variability of CBP within normal samples, to compare ciliary function in 3 profiles, and CBF calculated over 5 or 10 beat cycles.

Methods  Nasal brushing samples from 13 healthy subjects were recorded using DHSV in 3 profiles. CBP and CBF over 10 beat cycle were evaluated in all profiles and CBF re-evaluated over 5 beat cycles in the sideways edges.

Results  82.1% of edges exhibited a uniform CBP. In the sideways profile, uniformity within the edge was lower(uniform normal CBP: 69.1%(sideways), 97.1%(towards), 92.0%(above)), and dyskinesia was higher. Inter-observer agreement for dyskinesia was poor. CBF was not different between profiles(p=0.8097), or between 10 and 5 beat cycles(p=0.1126).

Conclusions  Our study demonstrates a lack of uniformity and consistency in manual CBP analysis of healthy samples, emphasizing the risk of automated CBP analysis in limited regions of interest(ROIs), and of single and limited manual CBP analysis. The towards and above profiles may be used to calculate CBF, but may be less sensitive for evaluation of ciliary dyskinesia and CBP. CBF can be measured reliably by evaluation of only 5 ciliary beat cycles.


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