Sleep Disorders: Sleep Disorders III |

Intermittent Hypoxia Aggravated Bleomycin-Induced Pulmonary Fibrosis in a Mouse Model of Sleep Apnea FREE TO VIEW

Lian Li, PhD; Fangyuan Ren, BA; Jie Cao, MD; Baoyuan Chen, MD
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Tianjin Medical University General Hospital, Tianjin, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A584. doi:10.1016/j.chest.2016.02.609
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SESSION TITLE: Sleep Disorders III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Obstructive sleep apnea (OSA) has recently been associated with cardiovascular/ cerebrovascular disease, metabolic disorders, cognitive dysfunction, as well as cancer. However, the association between OSA and pulmonary fibrosis has not been reported. The aim of this work was to study the association between intermittent hypoxia (IH) and pulmonary fibrosis, the effects of IH on pulmonary fibrosis and the interventional role of tempol in mice and cell cultures.

METHODS: C57BL/6J male mice were intratracheally injected with bleomycin. Then these animals were randomly distributed into three groups (n = 4 each): normoxia group, IH group, and tempol group (exposed to IH and treated with tempol). The degree of lung fibrosis was assessed by H&E stain. MLg lung fibroblast were exposed to normoxia, continuous hypoxia, IH, as well as IH after treatment with tempol. α-smooth muscle actin (α-SMA) and type I collagen (COLI) were measured by western blotting.

RESULTS: Pulmonary fibrosis area and the level of α-SMA in lung tissue significantly increased in IH group in mice. The levels of α-SMA and COLI significantly increased in IH group in cell culture. However, these effects induced by IH could be partially attenuated by tempol pretreatment.

CONCLUSIONS: These results suggest that IH promotes bleomycin-induced pulmonary fibrosis, which could be attenuated by tempol.

CLINICAL IMPLICATIONS: This experimental study prompted OSA may be associated with pulmonary fibrosis in clinical research, however, epidemiological surveys still need to be performed.

DISCLOSURE: The following authors have nothing to disclose: Lian Li, Fangyuan Ren, Jie Cao, Baoyuan Chen

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