Sleep Disorders: Sleep Disorders III |

Study on Endogenous Cannabinoid Receptors CB1 of Bone Metabolism in Chronic Intermittent Hypoxia Rat Model FREE TO VIEW

Bei Wang, PhD; Xiaoling Gao, PhD; Yanli Jia, MD
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The Second Hospital of Shanxi Medical University, Taiyuan, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A582. doi:10.1016/j.chest.2016.02.607
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SESSION TITLE: Sleep Disorders III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: By testing the level of serum tartrate resistant acid phosphatase (TRAP), observing the expression of endogenous cannabinoid system (ECs) CB1 receptor (CB1R) and the pathological changes of bone tissue, this study was to investigate the mechanism of CB1R during the bone damage caused by chronic intermittent hypoxia (CIH).

METHODS: 48 rats randomly were divided into six groups: four weeks (4W) and six weeks (6W) rats of the control groups (CG), 4W and 6W rats of intermittent hypoxia groups (IH), 4W and 6W rats of hypoxic intervention groups (HI) which were intraperitoneal injection with CB1 antagonist (rimonabant) by 1.5mg/kg/d (before modeling). Test animals had been performed after four weeks or six weeks. To detect the activity of serum TRAP by using enzyme-linked immunosorbent method (ELISA) and CB1R by immunohistochemistry. Morphological changes were observed by hematoxylin-eosin staining (HE).

RESULTS:1. In CG the bone cell morphology was normal and the trabecular structure was complete. The bone cells in IH group for four weeks were mild edema, inflammatory cells mild infiltration, trabecular bone quantity reduced; while for six weeks, they were severe significantly. In HI group they were milder than IH group at the same time point. 2. Compared with CG, in IH group TRAP and CB1R was higher, and with the duration of hypoxia it became more remarkable. Compared with IH group, TRAP in the HI group was lower at the same time point. The difference was statistically significant (p < 0.05). 3. Pearson correlation analysis showed that there was a positive correlation between the CB1R and the TRAP activity.

CONCLUSIONS:1. CIH really could lead to the disorder of ECS, also lead to the bone metabolic and bone structure abnormally. With the extension of hypoxic exposure time, the bone damage would be more seriously. 2. Using CB1R antagonist (rimonabant) intervention could reduce the ECS and the bone metabolic disorder of the OSAS in rats model. 3. CIH could enhance the expression of bone cells CB1R and cause damage to the bone tissue structure to some extent. 4. In OSAS animal models, there was a certain correlation between the level of TRAP and the CB1R in bone cells. It could be a judge target that OSAS could lead to bone metabolic disorder.

CLINICAL IMPLICATIONS: With the incidence of OSAS are increasing, endogenous cannabinoid system (ECs) receptors CB1 might be an effect on bone tissue which could help us to provide the new treatment thoughts of OSAS combined with the bone metabolism and structure disorders.

DISCLOSURE: The following authors have nothing to disclose: Bei Wang, Xiaoling Gao, Yanli Jia

discussing information about a product/procedure/technique that is considered research and is NOT yet approved for any purpose




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