SESSION TITLE: Sleep Disorders III
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To establish and validate a novel model of cultured cells for imitating intermittent hypoxia.
METHODS: In a chamber with experiment cabin and simulated air control cabin, we decided the frequency and duration of the intermittent hypoxia model according to the time of hypoxia and reoxygenation. The subcultured A549 cells were randomly divided into 7 groups, respectively named as control group (C); 6 h intermittent hypoxia group (6IH); 9 h intermittent hypoxia group (9IH); 6 h simulated air control group (6AC); 9 h simulated air control group (9AC); 4 h sustained hypoxia group (4SH); 6 h sustained hypoxia group (6SH). When the model establishment finished, the cellular morphology was observed under inverted microscope. The mRNA expression of HIF-1α was detected by real-time fluorescence quantitative PCR. The protein expression of HIF-1α was determined by immunohistochemistry.