0
Sleep Disorders: Sleep Disorders III |

Development of Cell Culture Model of Intermittent Hypoxia

Xiaohong Cai; Hongfang Mei; Hongxia Wang; Fangfang Hong; Liya Chen; Jing Lin
Author and Funding Information

Department of Pulmonolory, Second Affiliated Hospital & Yuying Children′s Hospital, Wenzhou Medical University, Wenzhou, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A579. doi:10.1016/j.chest.2016.02.604
Text Size: A A A
Published online

Extract

SESSION TITLE: Sleep Disorders III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To establish and validate a novel model of cultured cells for imitating intermittent hypoxia.

METHODS: In a chamber with experiment cabin and simulated air control cabin, we decided the frequency and duration of the intermittent hypoxia model according to the time of hypoxia and reoxygenation. The subcultured A549 cells were randomly divided into 7 groups, respectively named as control group (C); 6 h intermittent hypoxia group (6IH); 9 h intermittent hypoxia group (9IH); 6 h simulated air control group (6AC); 9 h simulated air control group (9AC); 4 h sustained hypoxia group (4SH); 6 h sustained hypoxia group (6SH). When the model establishment finished, the cellular morphology was observed under inverted microscope. The mRNA expression of HIF-1α was detected by real-time fluorescence quantitative PCR. The protein expression of HIF-1α was determined by immunohistochemistry.

First Page Preview

View Large
First page PDF preview

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543