Sleep Disorders: Sleep Disorders III |

The Expression of Macrophage Migration Inhibitory Factor in Obstructive Sleep Apnea-Hypopnea Syndrome Patients and Its Injury to Endothelial Cells FREE TO VIEW

Ping Yuan; Xinglin Gao, AAS
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Guangdong General Hospital, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A578. doi:10.1016/j.chest.2016.02.603
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SESSION TITLE: Sleep Disorders III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To study the expression of macrophage migration inhibitory factor (MIF) in obstructive sleep apnea hypopnea syndrom (OSAHS) patients and its injury to endothelial cells.

METHODS: According to the PSG test results, the objects of study (n = 71) were divided into control group (n = 20), mild OSAHS group (n = 19), moderate OSAHS group (n = 15), the severe OSAHS group (n = 17). The patient was extracted 4 ml fasting peripheral blood, we detect MIF in plasma. We separate peripheral blood mononuclear cell (PBMC) in control group and severe OSAHS patients, then PBMC cocultured with umbilical vein endothelial cells (HUVEC) 72 hours. The apoptosis of HUVEC was detected by flow cytometry as well as endothelial cell function index such as ET-1, NO, sICAM-1 and IL-6 with ELISA method.

RESULTS: MIF expression in control group and mild, moderate and severe OSAHS patients was respectively (25.65±8.01), (27.55 ±9.15), (31.42±13.93), (38.66±14.66) ng/ml, multiple-group analysis (F = 15.65, P<0.001), and the severe OSAHS group of MIF expression is increased than the control group (P<0.01). MIF with Apnea hypoventilation index (AHI) (r = 0.365, P = 0.008) and Oxygen index reduction (ODI) (r = 0.308, P = 0.308) were positively correlated, but MIF and lowest blood oxygen (r = 0.323, P = 0.323) showed a negative correlation. Endothelial cell apoptosis rate in control group and severe OSAHS group was respectively (2.94±1.018) %, (8.23±3.013) %, there was statistically significant difference, P<0.001. ET-1 in control group and severe OSAHS group was respectively (6.71±5.52), (9.88±4.79) pg/ml, there was no statistically significant difference, P = 0.141. sICAM-1 in control group and severe OSAHS group was respectively (10.895±7.641), (20.277±6.786) ng/ml, there was statistically significant difference, P = 0.014. NO in control group and severe OSAHS group was respectively (35.174±15.688), (25.085±5.151) umol/L, there was no statistically significant difference, P = 0.067.

CONCLUSIONS: MIF expression increased with the severity of disease and significantly elevated in severe OSAHS patients, and is closely related to the degree of disease severity and hypoxia. Apoptosis of endothelial cells in severe OSAHS Patients was obviously higher than that of control group, MIF may participate in the progress of injury.

CLINICAL IMPLICATIONS: MIF is maybe the target of immunotherapy in the inflammatory disease, including OSAHS, which provides another choice to the moderate and severe OSAHS patients who don't accept CPAP therapy.

DISCLOSURE: The following authors have nothing to disclose: Ping Yuan, Xinglin Gao

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