SESSION TITLE: Sleep Disorders III
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To study the expression of macrophage migration inhibitory factor (MIF) in obstructive sleep apnea hypopnea syndrom (OSAHS) patients and its injury to endothelial cells.
METHODS: According to the PSG test results, the objects of study (n = 71) were divided into control group (n = 20), mild OSAHS group (n = 19), moderate OSAHS group (n = 15), the severe OSAHS group (n = 17). The patient was extracted 4 ml fasting peripheral blood, we detect MIF in plasma. We separate peripheral blood mononuclear cell (PBMC) in control group and severe OSAHS patients, then PBMC cocultured with umbilical vein endothelial cells (HUVEC) 72 hours. The apoptosis of HUVEC was detected by flow cytometry as well as endothelial cell function index such as ET-1, NO, sICAM-1 and IL-6 with ELISA method.