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Signs and Symptoms of Chest Diseases: Cough |

Establishment of Chronic Cough Model in Guinea Pigs by Citric Acid Inhalation FREE TO VIEW

Xianghuai Xu, MD; Yu Li, MD; Qiang Chen, MD; Hanjing Lv, MD; Zhongmin Qiu, MD
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Tongji Hospital, Tongji University School of Medicine, Shanghai, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A543. doi:10.1016/j.chest.2016.02.567
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SESSION TITLE: Cough

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: The purpose of the study is to establish the chronic cough model in guinea pigs and investigate the pathogenesis and adaptation of cough reflex hypersensitivity, which may provide a methodological guarantee for the future therapy of chronic cough and the pharmacological researches on drugs used to treat chronic cough.

METHODS: Healthy conscious Guinea Pigs were subjected to inhalation of citric acid at 0.2 M (n=15), 0.4 M (n=15) and 0.8 M (n=15) for 3 min (twice daily). The cough reflex reactivity and TRPV1 expression in the airway mucosa were determined in these guinea pigs.

RESULTS: After inhalation of 0.4 M citric acid, the number of cough changed over time: the number of cough increased gradually soon after citric acid inhalation (baseline: 6.20±2.28) and peaked at 15 days (13.35±4.69) when the TRPV1 expression in the trachea and bronchus and P substance of the bronchoalveolar lavage fluid were also the highest. Thereafter, the number of cough reduced and reached a trough at 25 days (2.61±1.02). After inhalation of 0.2 M citric acid, the number of cough remained stable over time, and 3 animals died at 3, 11 and 17 days, respectively, after inhalation of 0.8 M citric acid.

CONCLUSIONS: Repeated inhalation of citric acid, an agonist of TRPV1, increases the number of cough, and further inhalation of citric acid leads to its reduction, suggesting the adaptation of cough reflex hypersensitivity. Inhalation of 0.4 M citric acid for 3 min twice daily for a total of 15 days is an optimal way to establish chronic cough animal model in healthy conscious guinea pigs.

CLINICAL IMPLICATIONS: Cough reflex hypersensitivity is a common characteristic of chronic cough.

DISCLOSURE: The following authors have nothing to disclose: Xianghuai Xu, YU Li, Qiang Chen, Hanjing Lv, Zhongmin Qiu

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