SESSION TITLE: Pulmonary Vascular Disease: PAH
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To clarify the possible mechanism of chronic cigarette smoke (CS)-induced pulmonary hypertension and furthermore provide effective targets for prevention and treatment.
METHODS: The effects of chronic CS on rat pulmonary arterial smooth muscle in vivo and nicotine treatment on rat PASMCs in vitro were investigated. A fluorescence microscope was used to measure the basal intracellular calcium concentration ([Ca2+]i) and store operated calcium entry (SOCE) in rat PASMC; The expression of transient receptor potential 1 (TRPC1) and transient receptor potential 6 (TRPC6) at both mRNA and protein levels in isolated distal PA was examinated using Western blot and real-time PCR.