Pulmonary Physiology: Pulmonary Physiology |

Reference Value of Induced-Sputum Cell Counts in Chinese Adult Population and Effects of Age, Gender FREE TO VIEW

Wei Luo; Qiaoli Chen; Ruchong Chen; Yanqing Xie; Hui Wang; KeFang Lai
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State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A483. doi:10.1016/j.chest.2016.02.503
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SESSION TITLE: Pulmonary Physiology

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To establish the normal reference range of total and differential cell counts in induced sputum of healthy adults in China, and to determine the impact of age and gender on sputum cell counts.

METHODS: A total of 185 healthy nonsmokers between 19 and 74 years old underwent induced sputum test with hypertonic saline (3%) inhalation. Sputum samples were collected for total and differential cell counts.

RESULTS: The qualified sputum samples were obtained from 153 subjects with a success rate of 82.7% (153/185). Mean age of subjects was 43 year old. The mean, median (95%CI) total cell counts was 2.1×106/g, 2.0×106/g (0.7-4.5×106/g), The mean, median (95%CI) differential cell counts were as follows: neutrophil 38.6%, 36.4% (8.9%-73.8%); macrophage 59.5%, 61.0% (24.7%-90.0%); eosinophil 0.6%, 0.3% (0.0%-2.0%); lymphocyte 1.2%, 1.0% (0.0%-4.5%). There was positive correlation between the percentage of neutrophil and eosinophil and age (r=0.658, r=0.404; P<0.001), while a negative correlation was found between percentage of macrophage and age (r =-0.660, P<0.001). The percentage of neutrophil, macrophage and eosinophil were significantly different among different age groups. The total cell counts and sputum cell counts were comparable between male and female.

CONCLUSIONS: This study successfully establishes normal reference range of induced sputum cell counts in Chinese healthy adults. Neutrophilic and eosinophilic counts increase by age.

CLINICAL IMPLICATIONS: This is the first large sample study concerning normal reference range of sputum total and differential cell counts in Chinese population. In addition, we demonstrated the airway inflammation in health adult was related to age, which resulted in the disparity of sputum cell counts in adults at different ages. The application of induced sputum test and the interpretation of the results in either clinical practice or research should take the age of subject into account.

DISCLOSURE: The following authors have nothing to disclose: Wei Luo, Qiaoli Chen, Ruchong Chen, Yanqing Xie, Hui Wang, KeFang Lai

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