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Procedures: Procedures: Bronchoscopy |

The Expression of VEGF in the Patient of Benign Central Airway Stenosis After Cryotherapy FREE TO VIEW

Kunyao Yu
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Peking University First Hospital, Beijing, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A431. doi:10.1016/j.chest.2016.02.449
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SESSION TITLE: Procedures: Bronchoscopy

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Benign central airway stenosis caused by a variety of reasons and has a high recurrence rate. The recurrence may associated with excessive epithelio-scarring which means a wrong wound-healing way. Cryotherapy is an important technology in interventional pulmonology. Some research shows that cryotherapy may affect the tissue's wound-healing. VEGF is a grow factor taking part in the wound-healing. This study is try to tell the role of VEGF in airway wound-healing and find the relationship between cryotherapy and the change of VEGF's expression.

METHODS: Patients who accepted endoscopic interventional treatment in Peking University First Hospital Resperatory Department and reserved biopsy specimen with the diagnosis of benign central airway stenosis is recruited to the study. Immunohistochemical analysis is used to detect the expression of VEGF. Wax blocks are used to isolate RNA and detect the level of VEGFA, the gene of VEGF, by Real-time RT-PCR. Normal tracheobronchial tissues, obtained from patients who had undergone surgical resection because of malignancy, were used as a control.

RESULTS: 33 patients were enrolled. 52 endoscopic interventional treatments were performed. There are 8 cases in the control group. The significantly increased expression of VEGF in stenosis group were found by immunohistochemical staining of all the 60 cases. Measure the intergraded optical density (IOD) in both group. In stenosis group, the IOD of VEGF is 12452.72 (891.08,33467.50), there are significant differences with control group. 9 patients accept cryotherapy treatment 2-8weeks before the biopsy. The VEGF level is higher in the cryotherapy group than patient do not accept cryotherapy before in IOD (53456.82 vs 5443.27, p=0.012). Real-time RT-PCR shows that the expression of VEGFA is significantly higher in stenosis group.

CONCLUSIONS: In the airway granulation tissue of benign central airway stenosis patients, the expressions of VEGF is significantly higher. The level of VEGF is higher in 2-8 weeks after cryotherapy.

CLINICAL IMPLICATIONS: VEGF is a grow factor associated with the vascular proliferation and may improve the tissue oxygen supply. The hypoxia is one of the reason of excessive scar formation. Maybe the cryotherapy can improve the tissue oxygen supply by up regulation of VEGF expression.

DISCLOSURE: The following authors have nothing to disclose: Kunyao Yu

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