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Occupational and Environmental Lung Diseases: Occupational and Environmental Lung Diseases |

The Role of Endoplasmic Reticulum Stress in Silica-Induced Apoptosis in Alveolar Macrophages

Yongbin Hu, PhD; Xia Wu, MA; Pinhua Pan, PhD
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Department of Pathology, Xiangya Hospital, Central South University, Changsha, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A415. doi:10.1016/j.chest.2016.02.431
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SESSION TITLE: Occupational and Environmental Lung Diseases

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To explore the role of Endoplasmic Reticulum Stress (ERS) and its mediated apoptosis in Alveolar Macrophages (AM) induced by silica.

METHODS: RAW264.7 cells were incubated with 200μg/ml silica for indicated times (0, 6, 12, 24, 48h). The morphological changes of cells were observed by transmission electron microscopy (TEM). Real-time PCR and Western blot were performed to detect the protein expression of BiP and CHOP. DAPI staining, Flow Cytometry (FCM) and Cleaved caspase-3 were used to measure apoptosis. In the prevention experiments, the ERS inhibitor 4-PBA was used to inhibit ERS and evaluate the changes of cell apoptosis.

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