Obstructive Lung Diseases: COPD II |

Aeca is Involved in the Alveolar Septal Cell Apoptosis Associated With Autoimmune Emphysema of Rats FREE TO VIEW

Xiang-yan Zhang; Cheng Zhang
Author and Funding Information

Guizhou Provincial People's Hospital, Guiyang, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A386. doi:10.1016/j.chest.2016.02.401
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: This study aimed at investigating the relationship between the AECA and alveolar septal cell apoptosis in autoimmune emphysema rats.

METHODS: Twenty four rats were randomly divided into three groups: a normal control group (n = 8), an autoimmune emphysema model group (n = 8) and an intervention group (n = 8). Intraperitoneal injection with primary cultured human umbilical vein endothelial cells was given to establish the autoimmune emphysema models, while the intervention group injected intraperitoneally with the same cells and methylprednisolone. The levels of AECA in bronchoalveolar lavage fluid (BALF) were determined using ELISA. Lung tissue section stained by HE was observed, Mean liner intercept (MLI) and mean alveolar numbers (MAN) measured. The expression of VEGFR-2 in the lung was detected using immunohistochemical analysis. TUNEL technique was used to detect the alveolar septal cell apoptosis.

RESULTS: The MLI, AI, AECA levels in BALF were higher in model group than those in normal control group and intervention group, while the MAN and VEGFR-2 expression was lower on the contrary (all P<0.05).

CONCLUSIONS: AECA may be involved in the alveolar septal cell apoptosis associated with the development of autoimmune emphysema of rats. Interventional effect of Methyprednislone on it results in increased VEGFR-2 expression and decreased AI to partially prevent the development of autoimmune emphysema of rats.

CLINICAL IMPLICATIONS: Use of Methyprednislone may induce increased VEGFR-2 expression and decreased AI to partially prevent the development of autoimmune emphysema

DISCLOSURE: The following authors have nothing to disclose: Xiang-yan Zhang, Cheng Zhang

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