SESSION TITLE: COPD II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To explore whether TLR3 is a risk factors in AECOPD have been challenged viruses.
METHODS: TLR3 were detected by Real-Time polymerase chain reaction and respiratory syncytial virus (RSV) were detected by nested reverse transcription polymerase chain reaction (RT-PCR) in lower respiratory tract specimens. 20 hospitalised patients with AECOPD and 10 Normal volunteers crowd with control admitted for other reasons were studied. Then TLR3 signaling pathway was explored in the lung epithelial cells. The A549 cells were infected by different congcentration of RSV in different time, and TLR3mRNA expression was detected by RT-PCR, protein expression of TLR3 and interferon regulatory factor 3 (IRF3) were detected by Western Blot, IRF3 protein localization was measured by immunofluorescence. Interferonβ (INF-β) and interleukin 6 (IL-6) were detected in cell supernatans by ELISA.