The Institute of Respiratory Diseases in Zunyi City, The First People's Hospital of Zunyi City, Zunyi, China
Copyright 2016, American College of Chest Physicians. All Rights Reserved.
SESSION TITLE: COPD II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To explore whether TLR3 is a risk factors in AECOPD have been challenged viruses.
METHODS: TLR3 were detected by Real-Time polymerase chain reaction and respiratory syncytial virus (RSV) were detected by nested reverse transcription polymerase chain reaction (RT-PCR) in lower respiratory tract specimens. 20 hospitalised patients with AECOPD and 10 Normal volunteers crowd with control admitted for other reasons were studied. Then TLR3 signaling pathway was explored in the lung epithelial cells. The A549 cells were infected by different congcentration of RSV in different time, and TLR3mRNA expression was detected by RT-PCR, protein expression of TLR3 and interferon regulatory factor 3 (IRF3) were detected by Western Blot, IRF3 protein localization was measured by immunofluorescence. Interferonβ (INF-β) and interleukin 6 (IL-6) were detected in cell supernatans by ELISA.
RESULTS: 4 of the 20 AECOPD patients sample had RSV detected. FEV1% lower in was detected RSV of AECOPD than was not detected RSV of AECOPD respectively, IL-6 is higher in was detected RSV of AECOPD than was not detected RSV of AECOPD. The AECOPD group showed a increase in TLR3mRNA compared with TLR3mRNA in the control, The difference in TLR3mRNA Increase between these two groups was significant. The RSV were dected AECOPD group showed a increase in TLR3mRNA compared with the RSV were not dected AECOPD group TLR3mRNA and showed a increase obviously compared with TLR3mRNA in the control. A significant relationship between severity of reductions in lung function at exacerbation and increase in sputum TLR3 of AECOPD by significant correlations. TLR3 signaling pathway was activated in the lung epithelial cells. TLR3 mRNA/protein increases compared with the control group in A549 with RSV infctiion. IRF3 protein also increases and the occurrence of nuclear transfer in A549 with RSV infctiion. INF-β and IL-6 both were increase compared with control in RSV infected A549 cells.
CONCLUSIONS: TLR3 detection in patients with AECOPD rises. It is associated with decline in the FEV1. TLR3 may be a risk factor for virus infection in AECOPD.
CLINICAL IMPLICATIONS: This study suggest that RSV may be a pathogenic factor for AECOPD and TLR3 inducing immune response may involve in the pathogenesis of COPD.
DISCLOSURE: The following authors have nothing to disclose: Shun Liu, Qian Chen, Yi Huang, Feng Wu, Ling Gong, Lan Zhu, Wei Zhang, Chuan Huang
No Product/Research Disclosure Information
Become a CHEST member and receive a FREE subscription as a benefit of membership.
Individuals can purchase this article on ScienceDirect.
Individuals can purchase a subscription to the journal.
Individuals can purchase a subscription to the journal or buy individual articles.
Learn more about membership or Purchase a Full Subscription.
Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.
Some tools below are only available to our subscribers or users with an online account.
Download citation file:
Web of Science® Times Cited:
Customize your page view by dragging & repositioning the boxes below.
Enter your username and email address. We'll send you a reminder to the email address on record.
Athens and Shibboleth are access management services that provide single sign-on to protected resources. They replace the multiple user names and passwords necessary to access subscription-based content with a single user name and password that can be entered once per session. It operates independently of a user's location or IP address. If your institution uses Athens or Shibboleth authentication, please contact your site administrator to receive your user name and password.