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Obstructive Lung Diseases: COPD II |

A Study of Respiratory Syncytial Virus-Toll Like Receptors 3-Mediated Immune Response and the Pathogenesis of Acute Exacerbations of Chronic Obstructive Pulmonary Disease

Shun Liu; Qian Chen; Yi Huang; Feng Wu; Ling Gong; Lan Zhu; Wei Zhang; Chuan Huang
Author and Funding Information

The Institute of Respiratory Diseases in Zunyi City, The First People's Hospital of Zunyi City, Zunyi, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A384. doi:10.1016/j.chest.2016.02.399
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SESSION TITLE: COPD II

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To explore whether TLR3 is a risk factors in AECOPD have been challenged viruses.

METHODS: TLR3 were detected by Real-Time polymerase chain reaction and respiratory syncytial virus (RSV) were detected by nested reverse transcription polymerase chain reaction (RT-PCR) in lower respiratory tract specimens. 20 hospitalised patients with AECOPD and 10 Normal volunteers crowd with control admitted for other reasons were studied. Then TLR3 signaling pathway was explored in the lung epithelial cells. The A549 cells were infected by different congcentration of RSV in different time, and TLR3mRNA expression was detected by RT-PCR, protein expression of TLR3 and interferon regulatory factor 3 (IRF3) were detected by Western Blot, IRF3 protein localization was measured by immunofluorescence. Interferonβ (INF-β) and interleukin 6 (IL-6) were detected in cell supernatans by ELISA.

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