SESSION TITLE: COPD II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To investigate the expressions of dendritic cells (DCs) and its chemokine receptor CCR6 in the lungs of rats with COPD and the effect of anti-CCL20 therapy on it.
METHODS: 18 Wistar rats were randomized into three groups: control, COPD model and CCL20 monoclonal antibody treated. In the latter two groups, COPD was induced by cigarette smoke exposure for four weeks and intratracheal LPS solution twice totally. CCL20 monoclonal antibody was injected intraperitoneally in the last group on the first day. All animals were sacrificed at the 29th day. Then pathomorphology of rats' lung and bronchiole were investigated by HE staining. The content of CCR6 in the BALF was analyzed with ELISA. DC distribution in rats' lung was evaluated by immunohistochemistry for OX62.