Obstructive Lung Diseases: COPD II |

The Standardized Herbal Formula, Bufei Granules, Alleviates Airway Mucus Hypersecretion in Mice With Chronic Obstructive Pulmonary Disease FREE TO VIEW

Sijia Guo, MD; Zengtao Sun, MD; Enshun Liu, MD; Jihong Feng, MD
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Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A381. doi:10.1016/j.chest.2016.02.396
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To observe the effects of Bufei granules on the airway mucus hypersecretion (AMH) in the mouse model of chronic obstructive pulmonary disease (COPD), and partly elucidate its mechanism.

METHODS: 80 C57BL/6J mice were randomly divided into 2 groups, blank control group (A) (n=20) and model group (B) (n=60). Mice were exposed to room air or cigarette smoke for 24 weeks. At the end of 24th week, 10 mice from each group were randomly selected to determine parameters, including the mouse pulmonary function (PF), the hematoxylin and eosin (H&E) staining sections, the alcian blue-periodic acid Schiff (AB-PAS) staining sections, the protein level and the mRNA expression level of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), phospho-NF-κB (p-NF-κB), mucin 5B (MUC5B) and mucin 5AC (MUC5AC) of lung tissues. Then the rest mice in group B were randomly and evenly divided into 5 sub-groups: blank control treatment (C), positive control treatment (D), low dose treatment (E), medium dose treatment (F) and high dose treatment (G). Afterwards by oral injection, the mice in group A and C were given by physiological saline, while the mice in group D, E, F and G were given by carbocisteine or different dose Bufei granules. The treatment lasted for 12 weeks. Finally we measured the same indexes as before.

RESULTS: At the end of 24th week, PF of mice in group B was reduced significantly. In the H&E and AB-PAS staining sections of lung tissues from mice in group B, emphysema and AMH were observed obviously. The protein level and the mRNA expression level of TLR4, NF-κB, p-NF-κB, MUC5B and MUC5AC of lung tissues of group B mice increased greatly. After treatment, comparing to group C, in group D, E, F and G carbocisteine and Bufei granules intervention improved PF, meanwhile alleviated emphysema and AMH of lung tissues. The higher the dose of Bufei granules is, the more significantly the effects do. The effects of high dose Bufei granules were similar to carbocisteine. Bufei granules intervention also lowered the protein level and the mRNA expression level of TLR4, NF-κB, p-NF-κB, MUC5B and MUC5AC of lung tissues from mice in group E, F and G.

CONCLUSIONS: Bufei granules could effectively alleviate AMH associated to COPD by regulating the signaling pathway of TLR4/NF-κB/MUC5B (MUC5AC).

CLINICAL IMPLICATIONS: This study provided a new choice to alleviate AMH of COPD, so as to improve the life quality, decrease the frequency of acute exacerbation and mortality of patients with COPD.

DISCLOSURE: The following authors have nothing to disclose: Sijia Guo, Zengtao Sun, Enshun Liu, Jihong Feng

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