0
Obstructive Lung Diseases: COPD: Etiology |

Angiotensin Receptor Blockade Attenuates Cigarette Smoke Extract-Induced Apoptosis of A549 Cells Through Up-Regulation of Nrf2 FREE TO VIEW

Ting Li, MD; Xu Li, PhD; Yan Chen, MD; Miaoxia Pan, MD; Liheng Guo, PhD; Ying Meng, PhD
Author and Funding Information

Department of Intensive Care Unit (ICU), Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A355. doi:10.1016/j.chest.2016.02.370
Text Size: A A A
Published online

SESSION TITLE: COPD: Etiology

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Cigarette smoke (CS) is a highly complex mixture and many of its components are known carcinogens, mutagens, and other toxic substances, whici is associated with an increased incidence of chronic obstructive pulmonary disease (COPD). In this study, we hypothesized that Irbesartan, an angiotensin receptor blockade, protected against cigarette smoke extract-induced apoptosis of A549 human lung carcinoma cells by inducing the expression of Nrf2.

METHODS: In vitro, A549 human lung carcinoma cells were pretreated with the angiotensin receptor blockade (Irbesartan) before exposure to cigarette smoke extract (CSE). The A549 human lung carcinoma cells were transfected with a small Nrf2-interfering RNA (Nrf2-siRNA) for suppressing endogenous Nrf2 expression before exposure to CSE and Irbesartan.

RESULTS: In vitro, CSE induced apoptosis of A549 cells and the apoptosis-related protein activation, including caspase3 and Bax. However, CSE suppressed the expression of Nrf2 protein and heme oxygenase-1 (HO-1) gene. Pretreatment with Irbesartan decreased CSE-induced cells apoptosis and the expression of caspase3 and Bax, but increased the expression of Nrf2 protein and HO-1 gene. However, these effects of Irbesartan were abolished by using a small Nrf2-interfering RNA (Nrf2-siRNA) transfection assay.

CONCLUSIONS: These data support our hypothesis that Irbesartan attenuates cigarette smoke extract-induced apoptosis of A549 cells through up-regulation of Nrf2.

CLINICAL IMPLICATIONS: Irbesartan may serve as a chemopreventive agent for the prevention and treatment of chronic obstructive pulmonary disease (COPD).

DISCLOSURE: The following authors have nothing to disclose: Ting Li, Xu Li, Yan Chen, Miaoxia Pan, Liheng Guo, Ying Meng

Irbesartan may serve as a chemopreventive agent for the prevention and treatment of chronic obstructive pulmonary disease (COPD).


Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543