Obstructive Lung Diseases: COPD: Etiology |

The Influence of Different Ways of Rearing and Tobacco Smoke Exposure to Rat’s Emphysema and the Atherosclerosis Formation and Interaction Research FREE TO VIEW

Hu Liu
Author and Funding Information

Shanxi Dayi Hostpital, Shanxi, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A354. doi:10.1016/j.chest.2016.02.369
Text Size: A A A
Published online


SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To explore the relationship of rat’s emphysema induced by different ways of rearing and tobacco smoke exposure and the atherosclerosis formation, and its possible mechanism.

METHODS: Ninety 6-week clean male SD rats [wight(200±20) g] were randomly divded into normal diet + tobacco smoke exposure group, fat-rich diet + tobacco smoke exposure group, limit intake + tobacco smoke exposure group, normal diet group, fat-rich diet group, and limit intake group. The relevant indices were measured in each groups after 24 weeks. Lee’s index was calculated. The serum TNF-α, IL-6 and adiponectin (APN) levels were detected by ELISA kits. Mean linear intercept (MLI) and mean alveoli number (MAN) were calculated and the aortic pathology change was observed by the microscope. Aortic vascular VEGF and BMP-2 levels were reflected by immunohistochemical method.

RESULTS: (1) Tobacco smoke exposure influenced MLI, MAN, aortic VEGF and BMP-2, Serum TNF-α, IL-6 and APN levels, tobacco smoke exposure made MLI, aortic VEGF and BMP-2, Serum TNF-α and IL-6 levels increasing, and MAN decreasing. (2) Different ways of rearing influenced MLI, MAN, aortic VEGF, Serum TNF-α, IL-6 and APN levels. In tobacco smoke exposure group: MLI in limit intake group was higher than it in fat-rich diet group and normal diet group. MAN in limit intake group was lower than it in fat-rich diet group and normal diet group. Aortic VEGF level in fat-rich diet group was higher than it in normal diet group and limit intake group. Serum TNF-α and IL-6 levels in limit intake group and fat-rich diet group were higher than those in normal diet group. Serum APN level in fat-rich diet group was lower than it in normal diet group and limit intake group, and it in normal diet group was lower than it in limit intake group. (3) In tobacco smoke exposure group, artic VEGF and BMP-2 levels had a positive correlation with MLI, serm TNF-α and IL-6 levels, and an inverse correlation with MAN level. Observating aorta by the microscopic, there was early atherosclersis in tobacco smoke exposure group and no atherosclerosis in non-smoking group.

CONCLUSIONS: Different ways of rearing and tobacco smoke exposure both can influence the formation of emphysema. There is a positive correlation between emphysema and atherosclerosis, and systemic inflammation may be involved.

CLINICAL IMPLICATIONS: Confirmed emphysema may promote atherosclerosis in animal experiments.

DISCLOSURE: The following authors have nothing to disclose: Hu Liu

No Product/Research Disclosure Information




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543