Obstructive Lung Diseases: COPD: Etiology |

Antitussive Effect of Schisandra Chinensis Extracts on Cigarette Smoke Exposed Guinea Pigs and Their Underlying Mechanism FREE TO VIEW

Li Yang; Shan Zhong; XiaoDong Liu; ChuQin Huang; KeFang Lai
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Guangzhou Institute of Respiratory Disease, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A353. doi:10.1016/j.chest.2016.02.368
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To compare the antitussive effect of different extracts of Schisandra chinensis on cough hypersensitive guinea pigs, and to further explore whether the antitussive effect of these extracts are relate to the inhibitions of airway neurogenic inflammation, oxidative stress factors and mRNA expression of the cough receptors (TRPV1 and TRPA1).

METHODS: ①Three extracts, namely petroleum ether extraction (PEE), ethyl acetate extraction (EAE), n-Butyl alcohol extraction (NBAE) and residue extraction (RE), were obtained from Schisandra chinensis by Polarity gradient extraction method. Guinea pigs exposed to successive periods of cigarette smoke (CS) for 14 days (twice a day, 10 cigarettes, 20 min in the morning or afternoon, respectively). During the modeling period, animals were daily oral administrated with three extracts respectively. Citric acid challenge and Biopsy specimens taken were followed in 15th and 16th day. ②A549 cells were proliferated for 24 h. The cells were incubated separately with various concentrations of schizandrin, schisantherin A, deoxyschizandrin, γ-schisandrin, 1 h followed by treatment with 2% CSE for another 1 h. The mRNA expression of TRPV1 and TRPA1 were detected by real time PCR.

RESULTS: Daily administration with PEE (41.1±9.7 times) or EAE (32.3±16.0 times) significantly inhibited CS exposure-enhanced cough, compared with guinea pig in model group (64.3±26.2 times); Daily treatment with EAE (1.25±0.59 x106/mL) significantly inhibited CS exposure-enhanced infiltration of total leukocytes and neutrophils, compared with guinea pig in model group (1.99 ± 0.44 x106/mL); Daily treatment with PEE or EAE significantly increased the activities of SOD and GSH-px, while remarkably reduced the content of MDA in lung tissue as compared to that in model group. Four lignins including schizandrin, schisantherin A, deoxyschizandrin, γ-schisandrin with concentration of 100 μM were remarkably reduced the CSE-induced mRNA expressions of TRPV1 and TRPA1.

CONCLUSIONS: PEE and EAE extract from Schisandra chinensis have significantly antitussive effect. The underlying mechanism might relate to anti-inflammation, antioxidation and decrease of mRNA expression of TRPV1 and TRPA1 in airway.

CLINICAL IMPLICATIONS: PEE and EAE extract from Schisandra chinensis might be a future antitussive drug for cough hypersensitive syndrome patients.

DISCLOSURE: The following authors have nothing to disclose: Li Yang, Shan Zhong, XiaoDong Liu, ChuQin Huang, KeFang Lai

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