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Obstructive Lung Diseases: COPD: Etiology |

Sulforaphane Suppressed LPS and Pam3CSK4 Mediated Inflammation in COPD Through MyD88-Dependent Toll-Like Receptors Pathway FREE TO VIEW

Xiaoli Zeng, MMed; Xiaoju Liu; Hairong Bao; Yi Zhang; Enli Tan
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The First Hospital of Lanzhou University, Lanzhou, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A351. doi:10.1016/j.chest.2016.02.366
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SESSION TITLE: COPD: Etiology

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Sulforaphane (SFN) is a natural isothiocyanate that is present in cruciferous vegetables such as broccoli and cabbage. Previous studies have shown that SFN is effective in preventing inflammation. The aim of this study is to test the the anti-inflammatory effect of SFN on LPS or Pam3CSK4-stimulated inflammation through inhibiting TLRs/MyD88 pathway in monocyte-derived macrophages (MDM) derived from chronic obstructive pulmonary disease (COPD) patients.

METHODS: MDM were obtained from healthy subjects and COPD patients and treated with the synthetic lipoprotein Pam3CSK4 or LPS in the absence or presence of SFN. The expressions of TLR2, TLR4 and MyD88 were determined by real-time polymerase chain reaction (PCR) and Western blot analysis. The inflammatory cytokines (interleukin 6, IL-6 and tumor necrosis factor α, TNF-α) in culture supernatants were measured by ELISA.

RESULTS: The baseline expressions of TLR2, TLR4 and MyD88 mRNA and protein and the downstream inflammatory cytokines in MDM patients from COPD patients were significantly higher compared to healthy controls. Furthermore, pretreatment of MDM with SFN significantly decreased Pam3CSK4 or LPS induced TLR2, TLR4 and MyD88 expression both at mRNA and protein levels along with decrease in the levels of IL-6, TNF-α.

CONCLUSIONS: These results suggest that SFN regulate inflammation in macrophages and may play a role in inflammatory diseases such as COPD.

CLINICAL IMPLICATIONS: Inflammation is a prominent feature of COPD, but there is no effective anti-inflammatory therapy for COPD. SFN, a dietary isothiocyanate derived from cruciferous vegetables, displays a variety of beneficial bioactivities through multiple mechanisms. Our study has demonstrated that SFN exerts anti-inflammatory activity through inhibiting the expression of cytokine through MyD88-dependent TLRs pathway in MDM from COPD patients. These new targets manipulated by SFN may provide its extensive perspective in clinical application.

DISCLOSURE: The following authors have nothing to disclose: Xiaoli Zeng, Xiaoju Liu, Hairong Bao, Yi Zhang, Enli Tan

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