Lung Cancer: Mechanisms of Lung Cancer |

Expression Profiles of Progesterone-Regulated Long Noncoding RNAs in Lung Adenocarcinoma Tumorigenesis and Progression FREE TO VIEW

Mingxuan Xie, MD; Qiong Chen, MD
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Xiangya Hospital, Central South University, Changsha, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A330. doi:10.1016/j.chest.2016.02.343
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SESSION TITLE: Mechanisms of Lung Cancer

SESSION TYPE: Original Investigation Slide

PRESENTED ON: Saturday, April 16, 2016 at 02:15 PM - 03:45 PM

PURPOSE: Lung adenocarcinoma has one of the highest morbidity and mortality rates of any cancer worldwide. Our previous studies have demonstrated that progesterone can inhibit lung adenocarcinoma A549 cells proliferation, migration and invasion in combination with Src inhibitor PP1, improve chemo-sensitivity to Gefitinib via a membrane progesterone receptor mechanism. Long noncoding RNAs (lncRNAs) have been shown to play essential roles in some types of cancer; however, the contributions of lncRNAs to progesterone-associated mechanisms in lung adenocarcinoma remain unknown.

METHODS: We used a high-throughput lncRNA microarray to compare the lncRNA and messenger RNA (mRNA) expression profiles in progesterone treated lung adenocarcinoma A549 cells and control groups. Several candidate progesterone-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis.

RESULTS: Using abundant and varied probes, we were able to assess 63 542 lncRNAs and 27 134 mRNAs in our microarray. We found that 2,102 lncRNAs and 1018 mRNAs were differentially expressed (2-fold change) in progesterone-treated lung adenocarcinoma A549 cells and control groups, indicating that many lncRNAs were significantly upregulated or downregulated in progesterone-associated process. We also found, via quantitative PCR, that 18 lncRNAs were aberrantly expressed in progesterone-treated lung adenocarcinoma A549 cells compared with control groups (p < 0.05).

CONCLUSIONS: By a microarray screening approach, 18 lncRNAs are aberrantly expressed in progesterone-treated lung adenocarcinoma A549 cells compared with control groups. They may play important roles in progesterone-regulated mechanisms in lung adenocarcinoma tumorigenesis and progression.

CLINICAL IMPLICATIONS: Our study is the first to ascertain the expression profiles of progesterone-regulated lncRNAs in lung adenocarcinoma A549 cells by microarray, suggesting that these lncRNAs may play a key role in progesterone-regulated lung adenocarcinoma tumorigenesis and progression. These may provide a new clue toward the development of early detection and novel promising targeted therapies for lung adenocarcinoma.

DISCLOSURE: The following authors have nothing to disclose: Mingxuan Xie, Qiong Chen

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