SESSION TITLE: Lung Cancer IV
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: This study was designed to predict non-small cell lung cancer metastasis and explore mechanism of lung cancer cell proliferation, metastasis and migration in vitro and vivo.
METHODS: Firstly, Adenylate cyclase-associated protein 1, CAP1 were determined by real-time PCR and western blot analysis and IHC in 82 lung cancer specimens. Secondly, BM group (n =50) and non-BM group (n =70) were treated and followed up by 4 years. Thirdly, we characterized the expression of CAP1 in lung cell by using Western blot and RT-PCR analyses. In addition, ShCAP1-LV, empty vector, CAP1-LV are cultured with A549, H1299, 95-D by using MTT, wound Healing, Transwell invasive, Western blot, and IHC analyses and nude mouse.