Lung Cancer: Lung Cancer IV |

Adenylate Cyclase-Associated Protein 1 Is Associated With Metastasis of Non-small Cell Lung Cancer Especially in Brain Metastasis Patients and Promotes the Lung Cancer Cell Proliferation and Migration In Vitro as Well as Its Growth and Metastasis In Vivo By the Signaling Pathways of Limk1-Cofilin FREE TO VIEW

Changhui Wang, PhD; Shuanshuan Xie, PhD; Xiaolian Song, PhD; Min Tan, MD
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Department of Respiratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A324. doi:10.1016/j.chest.2016.02.337
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: This study was designed to predict non-small cell lung cancer metastasis and explore mechanism of lung cancer cell proliferation, metastasis and migration in vitro and vivo.

METHODS: Firstly, Adenylate cyclase-associated protein 1, CAP1 were determined by real-time PCR and western blot analysis and IHC in 82 lung cancer specimens. Secondly, BM group (n =50) and non-BM group (n =70) were treated and followed up by 4 years. Thirdly, we characterized the expression of CAP1 in lung cell by using Western blot and RT-PCR analyses. In addition, ShCAP1-LV, empty vector, CAP1-LV are cultured with A549, H1299, 95-D by using MTT, wound Healing, Transwell invasive, Western blot, and IHC analyses and nude mouse.

RESULTS: We provided evidence that over-expression of CAP1 in NSCLC, particularly at BM. In addition, These findings suggest that CAP1 promotes the lung cancer cell proliferation and migration in vitro as well as its growth and metastasis in vivo via LIMK/cofilin I.

CONCLUSIONS: CAP1 is associated with metastasis of NSCLC especially in BM patients and promotes the lung cancer cell proliferation and migration in vitro and vivo by limk1-cofilin.

CLINICAL IMPLICATIONS: Lung cancer ranks first in both prevalence and mortality rates among all types of cancer. Metastasis is the main cause of treatment failure. Biomarkers are critical to early diagnosis and prediction and monitoring of progressive lesions. Several biomarkers have been identified for lung cancer but none have been routinely used clinically. The present study assessed the diagnostic and prognostic value of cyclase-associated protein 1 (CAP1) for lung cancer.

DISCLOSURE: The following authors have nothing to disclose: Changhui Wang, Shuanshuan Xie, Xiaolian Song, Min Tan

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