Lung Cancer: Lung Cancer IV |

Effects on Knocked Down Pokemonexpression on the Radiosensitivity of Pulmonary Adenocarcinoma Cells FREE TO VIEW

Yuan Niu, MMed; Jian Li, MMed; Hong Chen, MMed; Chun Zheng, MMed
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The First Affliated Hospital, Sun Yat-sen University, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A322. doi:10.1016/j.chest.2016.02.335
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To determine a new method for radiosensitization to treat pulmonary adenocarcinoma, we down-regulated Pokemon in pulmonary adenocarcinoma cells (A549).

METHODS: Three pairs of siRNA-knockdown Pokemon fragments from a eukaryotic expression vector, liposome-mediated transfection pulmonary adenocarcinoma cell line, A549 cells, underwent RT-PCR and western blotting before and after transfection Pokemon mRNA and protein expression screening, and silencing of best recombinant plasmid transfected A549 cells were observed in the A549 cells’ Pokemon gene expression by MTT cell proliferation assay, flow cytometry and apoptosis clonogenic assay, as well as in shRNA plasmid-transfected cells after combined radiation treatment and radiosensitization.

RESULTS: We successfully prepared shRNA fragments containing the recombinant plasmid-transfected A549 cells, which could significantly reduce Pokemon gene expression, reduce cell proliferation, accelerate apoptosis and increase A549 cells radiosensitivity.

CONCLUSIONS: Pokemon silencing could increase A549 cells radiosensitivity, providing a new radiosensitization paradigm for the clinical treatment of pulmonary adenocarcinoma.

CLINICAL IMPLICATIONS: Pokemon could be combined with radiotherapy, in the process engaging many other related cell signaling pathways, rather than simply interfering with the ARF-Pokemon pathway, for more significant results. The results of gene transduction combined with radiotherapy as a new clinical protocol could provide preliminary experimental evidence.

DISCLOSURE: The following authors have nothing to disclose: Yuan Niu, Jian Li, Hong Chen, Chun Zheng

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