0
Lung Cancer: Lung Cancer IV |

Effects on Knocked Down Pokemonexpression on the Radiosensitivity of Pulmonary Adenocarcinoma Cells FREE TO VIEW

Yuan Niu, MMed; Jian Li, MMed; Hong Chen, MMed; Chun Zheng, MMed
Author and Funding Information

The First Affliated Hospital, Sun Yat-sen University, Guangzhou, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A322. doi:10.1016/j.chest.2016.02.335
Text Size: A A A
Published online

SESSION TITLE: Lung Cancer IV

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To determine a new method for radiosensitization to treat pulmonary adenocarcinoma, we down-regulated Pokemon in pulmonary adenocarcinoma cells (A549).

METHODS: Three pairs of siRNA-knockdown Pokemon fragments from a eukaryotic expression vector, liposome-mediated transfection pulmonary adenocarcinoma cell line, A549 cells, underwent RT-PCR and western blotting before and after transfection Pokemon mRNA and protein expression screening, and silencing of best recombinant plasmid transfected A549 cells were observed in the A549 cells’ Pokemon gene expression by MTT cell proliferation assay, flow cytometry and apoptosis clonogenic assay, as well as in shRNA plasmid-transfected cells after combined radiation treatment and radiosensitization.

RESULTS: We successfully prepared shRNA fragments containing the recombinant plasmid-transfected A549 cells, which could significantly reduce Pokemon gene expression, reduce cell proliferation, accelerate apoptosis and increase A549 cells radiosensitivity.

CONCLUSIONS: Pokemon silencing could increase A549 cells radiosensitivity, providing a new radiosensitization paradigm for the clinical treatment of pulmonary adenocarcinoma.

CLINICAL IMPLICATIONS: Pokemon could be combined with radiotherapy, in the process engaging many other related cell signaling pathways, rather than simply interfering with the ARF-Pokemon pathway, for more significant results. The results of gene transduction combined with radiotherapy as a new clinical protocol could provide preliminary experimental evidence.

DISCLOSURE: The following authors have nothing to disclose: Yuan Niu, Jian Li, Hong Chen, Chun Zheng

No Product/Research Disclosure Information


Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543