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Lung Cancer: Lung Cancer IV |

Effects on Knocked Down Pokemonexpression on the Radiosensitivity of Pulmonary Adenocarcinoma Cells

Yuan Niu, MMed; Jian Li, MMed; Hong Chen, MMed; Chun Zheng, MMed
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The First Affliated Hospital, Sun Yat-sen University, Guangzhou, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A322. doi:10.1016/j.chest.2016.02.335
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SESSION TITLE: Lung Cancer IV

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To determine a new method for radiosensitization to treat pulmonary adenocarcinoma, we down-regulated Pokemon in pulmonary adenocarcinoma cells (A549).

METHODS: Three pairs of siRNA-knockdown Pokemon fragments from a eukaryotic expression vector, liposome-mediated transfection pulmonary adenocarcinoma cell line, A549 cells, underwent RT-PCR and western blotting before and after transfection Pokemon mRNA and protein expression screening, and silencing of best recombinant plasmid transfected A549 cells were observed in the A549 cells’ Pokemon gene expression by MTT cell proliferation assay, flow cytometry and apoptosis clonogenic assay, as well as in shRNA plasmid-transfected cells after combined radiation treatment and radiosensitization.

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