SESSION TITLE: Lung Cancer III
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: we will investigate the changes of iTreg function after the changing of T cell’ antigen avidity by NY-ESO-1 specific HAT, and study the effects of HAT engineered iTreg on the function of other immune cells.
METHODS: 1. Construct TCR lentiviral vectors with different affinity and pack lentivirus 2. Transduct and transfect different affinity-TCR genes into iTreg 3. Compare the difference of the ability between iTregs and HAT- iTreg (cytokine release, inhibition of antigen presentation and tumor cell killing) 4. Construct non-small cell lung cancer (NSCLC) animal model, and study the function of HAT- iTreg (tumor size, number of DC, NK, T cells in PBMC)