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Lung Cancer: Lung Cancer III |

A Investigation on the Effect of Promoting the Anti-Tumor Immunity of the DC Pulsed by CD40L+ Tumor Components FREE TO VIEW

Kun Tian, MD
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General Hospital of Chengdu Military Region of PLA, Chengdu, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A316. doi:10.1016/j.chest.2016.02.329
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SESSION TITLE: Lung Cancer III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To probe the exact role of the DC costimulated by CD40Len and homogenous tumor components promoting the anti-tumor immunity of response.

METHODS: DC induced for 7 days were sensitized with special sensitizing agents made of A549 after its freeze thawing after CD40L transfection. Whereafter, expression of the cell surface molecules (CD83, CD86, HLA—DR) on sensitized DC were detected with FCM, whose results were compared with that of empty vector group. Sensitized DC were co-cultured with homogenous admixture lymphocytes for 5 days after its inhibition with mitomycin, followed by measurement and comparison of lymphocytes proliferation of un-interfered group, empty group and transfection group with MTT assay. After its inhibition with mitomycin, sensitized DC were co-cultured with homogenous T cells and parental A549 cells at certain ratio for 72h, followed by measurement and comparison of T cell and A549 cells proliferation of un-interfered group, empty group and transfection group with MTT assay.

RESULTS: After they were sensitized by prepared A549 cells expressing CD40L, DC cells had higher expression of surface molecules such as CD86, HLA—DR (MHC-Ⅱ) compared with that in empty vector group with statistical significance (P86=0.013<0.05, PHLA-DR=0.036<0.05). The expression of CD83 was higher than that in empty vector grou (P=0.06>0.05), but there had not been any statistical significance so far. 2) Compared to un-interfered group and empty vector group, DC in CD40L transfected group had greater ability in mediating the multiplication of homogeneous lymphocytes and the inhibition on proliferation of parental A549 cells after they were sensitized by sensitizers with statistical significance (P<0.05).

CONCLUSIONS: Compared to DC sensitized by tumor cells with no expression of CD40L, DC had obvious higher expression rate of costimulatory molecules and greater greater ability in mediating the multiplication of homogenous lymphocytes as well as the growth inhibition on homogenous tumor cells when they were sensitized by prepared tumor cells with expression of transfected CD40L.

CLINICAL IMPLICATIONS: This result provide powerful experiment backing for immunotherapy of lung carcer in clinic.

DISCLOSURE: The following authors have nothing to disclose: Kun Tian

The Presenter is a MD since working she has gone in for clinical diagnose and treatment of lung carcinoma in General Hospital of Chengdu Military Region of PLA carried out this study of “A investigation on the effect of promoting the anti-tumor immunity of the DC pulsed by CD40L+ tumor components” simultaneously. The technique of cell culture, recombination of gene and FCM were been utilized in the experiment.


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