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Lung Cancer: Lung Cancer III |

A Investigation on the Effect of Promoting the Anti-Tumor Immunity of the DC Pulsed by CD40L+ Tumor Components

Kun Tian, MD
Author and Funding Information

General Hospital of Chengdu Military Region of PLA, Chengdu, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A316. doi:10.1016/j.chest.2016.02.329
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SESSION TITLE: Lung Cancer III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To probe the exact role of the DC costimulated by CD40Len and homogenous tumor components promoting the anti-tumor immunity of response.

METHODS: DC induced for 7 days were sensitized with special sensitizing agents made of A549 after its freeze thawing after CD40L transfection. Whereafter, expression of the cell surface molecules (CD83, CD86, HLA—DR) on sensitized DC were detected with FCM, whose results were compared with that of empty vector group. Sensitized DC were co-cultured with homogenous admixture lymphocytes for 5 days after its inhibition with mitomycin, followed by measurement and comparison of lymphocytes proliferation of un-interfered group, empty group and transfection group with MTT assay. After its inhibition with mitomycin, sensitized DC were co-cultured with homogenous T cells and parental A549 cells at certain ratio for 72h, followed by measurement and comparison of T cell and A549 cells proliferation of un-interfered group, empty group and transfection group with MTT assay.

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