The Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an, China
Copyright 2016, American College of Chest Physicians. All Rights Reserved.
SESSION TITLE: Lung Cancer II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: Predict and identify the HLA-A*0201 restricted CTL epitopes with the strongest immunogengenicity of SOX2 antigen.
METHODS: Predict and screen the natural epitopes with bioinformatics method. Synthesize peptides in vitro. T2 cells were induced by peptides, and then were used in T2 binding assay. The effective cells were acquired by inducing PBMCs with peptides, and the target cells were acquired by inducing T2 cells with peptides. Then they were used in ELISPOT hIFN-γ and LDH releasing tests.
RESULTS: Natural epitopes were obtained: SMYLPGAEV (P1), TLMKKDKYT (P2) and ALGSMGSVV (P3). The binding force between P1 and MHC I increased with the increase of the concentration of peptide, and when the concentration was 50μmol/L and 100μmol/L P1 group was the highest. Effect of killing the target cells can be observed in P1 and P2 group, and the former can secrete more IFN-γ.
CONCLUSIONS: SMYLPGAEV is the strongest HLA-A*0201 restricted CTL natural epitope of SOX2 antigen, and may induce a strong immune response in patients with tumor.
CLINICAL IMPLICATIONS: This peptide may play an important role in tumor vaccine.
DISCLOSURE: The following authors have nothing to disclose: Yu Wang, Jingyan Yuan, Jingyuan Xiao, Wei Li, Na Fan, Wenjing Deng, Boxuan Liu, Ping Fang, Yujie Zhong, Shuanying Yang
No Product/Research Disclosure Information
Become a CHEST member and receive a FREE subscription as a benefit of membership.
Individuals can purchase this article on ScienceDirect.
Individuals can purchase a subscription to the journal.
Individuals can purchase a subscription to the journal or buy individual articles.
Learn more about membership or Purchase a Full Subscription.
Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.
Some tools below are only available to our subscribers or users with an online account.
Download citation file:
Web of Science® Times Cited:
Customize your page view by dragging & repositioning the boxes below.
Enter your username and email address. We'll send you a reminder to the email address on record.
Athens and Shibboleth are access management services that provide single sign-on to protected resources. They replace the multiple user names and passwords necessary to access subscription-based content with a single user name and password that can be entered once per session. It operates independently of a user's location or IP address. If your institution uses Athens or Shibboleth authentication, please contact your site administrator to receive your user name and password.