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Lung Cancer: Lung Cancer II |

Effects and Mechanisms of lncRNA RP11-366L20.3 on Non-small Cell Lung Cancer Metastasis

Shuanying Yang, PhD; Yujie Zhong, MMed; Xuemei Zhang, PhD; Wei Li, MMed; Zongjuan Ming, MMed; Yuping Zhang, MMed; Wenjing Deng, MMed; Hongyang Shi, MMed; Na Fan, MMed; Zequn Niu, MMed; Xia Meng, MB; Zongfang Li, PhD; Chen Huang, PhD
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The Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A307. doi:10.1016/j.chest.2016.02.320
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SESSION TITLE: Lung Cancer II

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: This study aimed to screen NSCLC metastasis associated lncRNAs and explore the effects and mechanisms of objective lncRNA in NSCLC metastasis.

METHODS: LncRNA chip technology was employed to screen the differentially expressed lncRNAs between high metastatic cell line 95D and low metastatic cell line 95C. A stable 95D cell line that silenced the expression of objective lncRNA was constructed using lentiviral interference technology. MTT, transwell assay, adhesion assay and electron microscopy were used to detect changes in cell proliferation, migration, invasion, adhesion ability and cell shape. QPCR, flow cytometry, Zymogram Gels and Western blot identified NSCLC metastasis related genes and proteins. Using the nude mice model, size of the primary tumor was recorded and expressions of proteins were evaluated.

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