Lung Cancer: Lung Cancer II |

Inhibition Effects of Metformin and Pemetrexed on the Proliferation, Apoptosis and Migration Capabilities of Non-small Cell Lung Cancer Cell Line A549 in vitro FREE TO VIEW

Yan Zhang, MA
Author and Funding Information

QiLu Hospital of ShanDong, Jinan, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A305. doi:10.1016/j.chest.2016.02.318
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To investigate the effects of metformin and pemetrexed on the proliferation, apoptosis and migration capabilities of non­small cell lung cancer cell A549 in vitro.

METHODS: After A549 cells were treated with metformin and pemetrexed alone, or in combination for different period (24,48,72 h), the inhibition of cell proliferation rate in each groups was measured by CCK8, apoptosis rate was texted by flow cytometry and we can see the different migration capabilities by Wound scratch assay.

RESULTS: Compared with the control group (A549cells without any treatment), the proliferative inhibition rate of A549 cells in combination of metformin and pemetrexed group were higher than those in metformin or pemetrexed alone group (P < 0.05). By contras, the apoptosis rate of combination groups was increased more obviouslythan the contral and monotherapy groups, which had a synergistic effect.

CONCLUSIONS: The combination of metformin and pemetrexed generates a synergistic effect on cell proliferation, apoptosis and migration capabilities in A549 cells in vitro.

CLINICAL IMPLICATIONS: Improve chemotherapy in patients with efficient, prolong life, improve the quality of life.

DISCLOSURE: The following authors have nothing to disclose: Yan Zhang

FCM (flow cytometry) could use special instrument to mesure the apoptosis rate of A549 cell with different disposions.




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