SESSION TITLE: Lung Cancer II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To study the effect of cell proliferation and apoptosis in A549 lung cancer cells and to find out the influence on other key enzymes of metabolism.
METHODS: With three chemosynthesis siRNA Duplex targeting transketolase (TKT), A549 lung cancer cells were cultured and transfected. One of the best siRNA with highest interference effect was selected by fluorogenic Quantitative PCR. Transfection cells of 48h and 72h were detected by Flow Cytometry for cell cycle and apoptosis and by fluorogenic Quantitative PCR to record the changes of other metabolic key enzymes mRNA.