Lung Cancer: Lung Cancer II |

miRNA-204 Suppresses Human Non-small Cell Lung Cancer by Targeting ATF2 FREE TO VIEW

Shuo Zhang; Hui Ren, MD; Mingwei Chen
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The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A300. doi:10.1016/j.chest.2016.02.313
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: The role of microRNAs (miRNAs) in development and progression of cancer is an established fact. Dysregulated expression of miR-204 has been testified in several cancers, but the mechanism by which miR-204 modulates human non-small cell lung cancer (NSCLC) is largely unknown.

METHODS: In this study, we investigated the expression and functional role of miR-204 in human NSCLC tissues and cell lines. RNA isolation, qRT-PCR, MTT, colony formation assay, cell cycle assay, cell apoptosis assay, cell migration assay and Western blot were done. Statistical analysis was performed using SPSS 18.0 software and a two-sided p value < 0.05 was considered the threshold for statistical significance.

RESULTS: miR-204 level was significantly decreased in NSCLC tissues as compared with their respective non-neo plastic counterparts. Transient over expression of miR-204 by transfecting with miR-204 mimics suppressed NSCLC cell proliferation, migration and induced apoptosis and G1 arrest, whereas inhibition ofmiR-204 presented the reverse trend. Moreover, ATF2, an important transcription factor, was predicted the target gene of miR-204. Subsequent investigation found a negative correlation between miR-204 levelandATF2 expression in NSCLC tissue samples. Furthermore, we observed that miR-204 expression inversely affected endogenous ATF2 expression at both mRNA and protein levels in vitro.

CONCLUSIONS: Taken together, miR-204acts as a tumor suppressor by directly targeting ATF2 in NSCLC.

CLINICAL IMPLICATIONS: It helps us to well understand the mechanism of lung cancer process and provides a strategy for precise medicine.

DISCLOSURE: The following authors have nothing to disclose: Shuo Zhang, Hui Ren, Mingwei Chen

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