SESSION TITLE: Mechanisms of Infections
SESSION TYPE: Original Investigation Slide
PRESENTED ON: Sunday, April 17, 2016 at 04:00 PM - 05:00 PM
PURPOSE: In this study, we sought to determine the correlation between the degree of serum VDBP and progression of sepsis patients and mouse sepsis model.
METHODS: We performed a prospective study on 131 patients diagnosed with sepsis at different stages (sepsis group) and 25 patients with severe acute pancreatitis (control groups). A classic caecal ligation and puncture (CLP) in comparison with a sham procedure was used to establish experimental sepsis model in mice (n=40). Serum VDBP levels were assayed by ELISA. The pathologic changes were observed under electron microscope via HE stain. To measure the optical density of VDBP immunoreactivity, the region of interest in the lung, liver and spleen of mice were acquired.