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Critical Care: Critical Care: Sepsis |

LPS-Induced Mitochondrial DNA Release Causes Acute Lung Injury and Systemic Inflammation Through Toll-like Receptor 9 in Mice

Songyun Deng; Yuhang Ai; Lemeng Zhang; Pinhua Pan; Dongdong Wu
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Department of Intensive Care Unit, Xiangya Hospital, Changsha, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A168. doi:10.1016/j.chest.2016.02.174
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SESSION TITLE: Critical Care: Sepsis

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To investigate the role of mitochondrial DNA (mtDNA) release on lipopolysaccharide (LPS) induced acute lung injury (ALI) and systemic inflammation.

METHODS: Wild type C57BL/6 mice and matched toll-like receptor 4 (TLR4) KO mice were randomly assigned to sham and LPS groups. LPS/PBS were given by intraperitoneal (IP) injection. The mice were sacrificed 16 hours after LPS injection and plasma were isolated for mtDNA detection by Q-PCR. Furthermore, C57BL/6 mice were randomly assigned to ODN 2088 control and ODN2088 group pretreated 1h before mtDNA injection; all the mice were sacrificed 16 hours later. Lungs were harvested for HE staining; and were for BALF total protein concentration and lung W/D ratio analyse. Serum levels of IL-1 beta, IL-6, HMGB1 were measured by ELISA kit.

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