SESSION TITLE: Critical Care: Sepsis
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To investigate the role of mitochondrial DNA (mtDNA) release on lipopolysaccharide (LPS) induced acute lung injury (ALI) and systemic inflammation.
METHODS: Wild type C57BL/6 mice and matched toll-like receptor 4 (TLR4) KO mice were randomly assigned to sham and LPS groups. LPS/PBS were given by intraperitoneal (IP) injection. The mice were sacrificed 16 hours after LPS injection and plasma were isolated for mtDNA detection by Q-PCR. Furthermore, C57BL/6 mice were randomly assigned to ODN 2088 control and ODN2088 group pretreated 1h before mtDNA injection; all the mice were sacrificed 16 hours later. Lungs were harvested for HE staining; and were for BALF total protein concentration and lung W/D ratio analyse. Serum levels of IL-1 beta, IL-6, HMGB1 were measured by ELISA kit.