Critical Care: Critical Care: ARDS/ALI |

Effects of Vaporized Perfluorocarbon on Surfactant Proteins in an Animal Model of LPS-Induced Acute Lung Injury FREE TO VIEW

BingChao Han, DrPH; Kun Xiao; LongXiang Su; Youning Liu; Lixin Xie
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Department of Respiratory Disease, Chinese PLA General Hospital, Beijing, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A154. doi:10.1016/j.chest.2016.02.160
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Vaporized perfluorocarbon has been shown to improve gas exchange and pulmonary mechanics in acute lung injury. However, the mechanism of action of this technique is still poorly understood. The authors tested the hypothesis that administration of vaporized perfluorocarbon may improve surfactant proteins synthesizing in LPS-induced acute lung injury.

METHODS: Twenty-four famale New Zealand White rabbits were ventilated in a volume-controlled mechanical ventilator and randomly divided into four groups: (1) the ALI+PFC group received conventional mechanical ventilation and vaporized PFC inhalation after acute lung injury was induced by intravenous injection of LPS; (2) the ALI group received conventional mechanical ventilation after ALI animal models were established; (3) the PFC group received conventional mechanical ventilation and vaporized PFC inhalation; (4) the Control group only received conventional mechanical ventilation. The animals in the ALI+PFC and ALI groups received vaporized PFC inhalation for 2 hours followed by conventional ventilation 6 hours. The venous blood and arterial blood were extracted every hour during the experiment. After completion of the study, the animals were killed and lung tissue samples were taken for analysis. We evaluated surfactant proteins in plasma, bronchial alveolar lavage fluids (BALF) and lung tissue by ELISA, real-time PCR or Western Blot.

RESULTS: Plasma analysis revealed that SP-A, SP-B, SP-C and SP-D in the ALI+PFC were lower than in the ALI from the beginning of vaporized perfluorocarbon until the end of the experiment. The measurement of the BALF and lung tissues showed that SP-A, SP-B and SP-C were significantly increased in the ALI+PFC as compared with the ALI. However, Western blot results suggested that SP-D was lower in the ALI+PFC than in the ALI group. Among all the results about SP-A, SP-B, SP-C and SP-D, no differences were seen between the PFC and the Control.

CONCLUSIONS: The authors conclude that the administration of vaporized perfluorocarbon promotes the synthesis of SP-A, SP-B and SP-C and inhibits them from releasing into the blood in LPS-induced acute lung injury.

CLINICAL IMPLICATIONS: The present results revealed that promoting the synthesis of SP-A, SP-B and SP-C may be one of the mechanisms that vaporized perfluorocarbon improved gas exchange and pulmonary mechanics in acute lung injury.

DISCLOSURE: The following authors have nothing to disclose: BingChao Han, Kun Xiao, LongXiang Su, Youning Liu, Lixin Xie

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