Chest Infections: Chest Infections: Bronchieactasis |

Differences of Cytokine Levels in Bronchoalveolar Lavage Samples in Adults With Non-CF Bronchiectasis FREE TO VIEW

Evangelos Balis, DSc; Eugenios Metaxas, MD; Joseph Papaparaskevas, DSc; Georgios Tatsis, DSc; Nikos Spanakis, DSc; Athanasios Tsakris, DSc; Constantina Tsakanika, MD
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Pulmonary Department, Evangelismos General Hospital, Athens, Greece

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A100. doi:10.1016/j.chest.2016.02.105
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SESSION TITLE: Chest Infections: Bronchieactasis

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: The role of different cytokines in the pathogenesis of bronchiectasis and bronchiectasis exacerbations remains unclear. We endeavored to examine and compare the concentration of different cytokines from Bronchoalveolar Lavage (BAL) samples, obtained from patients with non-Cystic Fibrosis bronchiectasis.

METHODS: A cohort study with patients suffering from non-CF bronchiectasis was designed and performed. Patients were followed up for 12 months during clinically stable and exacerbation periods. During visits, they were submitted to bronchoscopy where BAL samples were obtained from the most affected lobe. The same time, blood samples were also obtained. Using the BDTM Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit, Interleukin-8 (IL-8), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interleukin-12p70 (IL-12p70) protein levels were quantitatively measured from the BAL samples. White Blood Count (WBC) and C-Reactive Protein (CRP) levels from the blood samples were also measured. We compared the mean values of these cytokines during exacerbation periods and during periods of clinical stable disease.

RESULTS: All cytokines, IL-8 (p=0.006), IL-6 (p=0.007), IL-10 (p=0.007), TNF (p=0.028) and IL-12p70 (p=0.019), except IL-1β (p=0.608), were statistically higher in exacerbation periods compared to clinical stable periods. We had the same results when we compared the cytokine levels between patients with positive or negative BAL cultures. All cytokines, IL-8 (p=0.001), IL-6 (p=0.003), IL-10 (p=0.001) TNF (p=0.002) and IL-12p70 (p=0.0006), except IL-1β (p=0.241), were statistically higher in patients with positive BAL cultures.

CONCLUSIONS: Cytokines in BAL samples are increased in patients with bronchiectasis during periods of exacerbations, regardless if they had positive or negative cultures. Furthermore, patients with positive BAL culture had higher cytokines levels comparatively with patients with negative cultures in all periods.

CLINICAL IMPLICATIONS: Associating inflammatory factors with the cause of exacerbation as well as with inflammatory factors in the blood and spirometric indices, would help to understand better the bronchiectasis inflammation.

DISCLOSURE: The following authors have nothing to disclose: Evangelos Balis, Eugenios Metaxas, Joseph Papaparaskevas, Georgios Tatsis, Nikos Spanakis, Athanasios Tsakris, Constantina Tsakanika

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