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Chest Infections: Chest Infections: Bacterial |

Establishment and Clinical Application of a Liquid Chip Technology for High Throughput Screening Pathogens of Acute Contagious Respiratory Disease FREE TO VIEW

Yusheng Chen; Hongru Li; Wei Zhang; Zhidan Hua; Xiaohong Lin; Mengqing Lin; Wensen Huang; Liping Huang; Xiaoli Yu; Nengluan Xu; Baosong Xie; Xiaona Shen; Jianfeng Xie; Yi Wang; Meng Huang; Yanan Wu; Xinglan Hu
Author and Funding Information

Fujian Provincial Hospital, Provincial Clinical Medical College, Fujian Medical University, Fuzhou City, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A94. doi:10.1016/j.chest.2016.02.099
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SESSION TITLE: Chest Infections: Bacterial

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To establish a liquid microarrays technology for the simultaneous, rapid, high throughput screening of 20 acute contagious respiratory disease pathogens. This technology can detect virus, atypical pathogens and bacteria simultaneously and rapidly. What more, This research was also designed investigate the clinical application value of this technology.

METHODS: 1:Establishment of the liquid chip technology:First, we obtain pathogens gene sequence of bacteria and atypical pathogens. The primers and the probes were designed on conserved sequence. Then, Pathogens’ gene was amplified. Amino-modified probes were spotted on encoding microbeads hybridized with the PCR amplicon. Finally, Liquid chip equipment get mean fluorescent intensity. As a result, The liquid chip technology can detecte the corresponding pathogens with these specific primers and the probes repeatedly and sensitivitily. 2: we used the liquid chip technology to screen 20 pathgens from 333 chinical speum samples, comparing with bacteria culture for bacteria and Real-time PCR assay for virus and atypical pathogens, so as to obtian the sensitivity and specificity of this method.

RESULTS: A liquid microarrays technology for high throughput screening of 20 contagious respiratory disease pathogens was establish sucessfully. Of 333 specimens, the positive rates of bacteria detection by liquid chip technology was significantly higherly than traditional bacteria culture (37.8%vs 10.8%) (P<0.05); The positive rates of atypical pathogens and viral detection by liquid chip technology and real-time PCR showed no significant differences (2.7%vs 3.0%) and (34.5% vs 31.2%) (P>0.05). comparing with standard methods, the sensitivity and specificity of liquid chip technology was 74.4% and 93.0% in bacteria detection, 50.0% and 99.4% in atypical pathogens detection, and 63.0% and 98.3% in viral detection.

CONCLUSIONS: A liquid chip technology for high throughput screening of pathogens of acute contagious respiratory disease including bacteria, virus and atypical pathogens was established. This technology has a potential clinical application in high throughput screening multiple species of repiratory pathgens of the unexplained acute pneumonia comparing with other methods before which can only screen Single species.

CLINICAL IMPLICATIONS: The liquid chip technology has a potential clinical application in high throughput screening multiple species of repiratory pathgens of the unexplained acute pneumonia comparing with other methods before which can only screen Single species.

DISCLOSURE: The following authors have nothing to disclose: Yusheng Chen, Hongru Li, Wei Zhang, Zhidan Hua, Xiaohong Lin, Mengqing Lin, Wensen Huang, Liping Huang, Xiaoli Yu, Nengluan Xu, Baosong Xie, Xiaona Shen, Jianfeng Xie, Yi Wang, Meng Huang, Yanan Wu, Xinglan Hu

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