Chest Infections: Chest Infections: Bacterial |

The Influence of Azithromycin to Triggering Receptor Expressed on Myeloid Cell-1 in the Pseudomonas aeruginosa Model of Rat FREE TO VIEW

Tong Jin
Author and Funding Information

The Second Hospital Affiliated Chongqing Medical University, Chongqing, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A92. doi:10.1016/j.chest.2016.02.097
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SESSION TITLE: Chest Infections: Bacterial

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To research the regulation of azithromycin to triggering receptor expressed on myeloid cell-1 (TREM-1) on the Pseudomonas aeruginosa (P.a) model of rat, and the effect to the life state, bacterial burden and inflammatory reaction on the model.

METHODS: The male S/D rats were separated to three groups: the control group, the azithromycin group and the TREM-1/Fc fusion protein group. The live time, survival amount and survival state were observed. The concentration of sTREM-1, TNF-α, IL-6 and IL-1β in serum were detected by ELISA every day. The bacterial burdens were determined by MTT. The inflammation damage level of lung tissues was manifested by hematoxylin and eosin (HE) stain.

RESULTS: In survival rate of rats, the TREM-1/Fc fusion protein group was the best, the azithromycin group was better and the control group was the worst. In the effect of inflammation factors in serum, the TREM-1/Fc fusion protein group was better than the azithromycin group, including sTREM-1, TNF-α (P<0.01), IL-6 and IL-1β (P<0.05). The concentrations of inflammation factors of the both groups were lower than it of the control group with significant deviation (P<0.01). The bacterial burden of the azithromycin group was minimums (P<0.01), it of the control group was maximum, and it of the TREM-1/Fc fusion protein group was between the other groups. In pathology injuries of lung tissue, the inflammation injuries of the TREM-1/Fc fusion protein group and the azithromycin group were better than it of the control group and it of the TREM-1/Fc fusion protein group was the best.

CONCLUSIONS: Azithromycin could inhibit the expression of TREM-1, relieve the inflammation injuries of lung tissue in the P.a model of rat, decrease the bacterial burden, extend live time and improve prognos

CLINICAL IMPLICATIONS: To confirm the low-dosage azithromycin could reduse the level of inflammation of sepsis.

DISCLOSURE: The following authors have nothing to disclose: Tong Jin

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