Chest Infections: Chest Infections: Bacterial |

A Combination of Small Protein a (SmpA) and Phospholipase D (PLD) Immunization Plays a Protective Role in Mice Pneumonia Model of Acinetobacter baumannii Infection FREE TO VIEW

Haitao Li; Pinhua Pan, MD
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Xiangya Hospital, Central South University of China, Changsha, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A89. doi:10.1016/j.chest.2016.02.094
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SESSION TITLE: Chest Infections: Bacterial

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Acinetobacter baumannii (Ab) is a global emerging bacterium resulting in nosocomial infections such as pneumonia, meningitis, bacteremia especially in intensive care units. Since Ab is resistant to almost all conventional antibiotics, the development of vaccine is one of the most promising and cost-effective strategies to prevent infections. In this study, we sought to investigate whether small protein A (SmpA) and Phospholipase D (PLD) were potential target to elicit protective immunity against A. baumannii infections.

METHODS: After schedule immunized with antigens in dose gradient, the IgG titer in serum were measured to obtain an appropriate dose for vaccine. The mice induced to diabetic models were divided into 4 groups immunized SmpA, PLD, SmpA and PLD separately in optimal dose, and one group as a control. All mice received A. Baumannii aerosolized infection after immunization. Then serum and lung tissue were harvested to identify the efficacy of the active immunization. Survival cure was observed in mice received immunized serum following with a lethal infection to identify the efficacy of the passive immunization.

RESULTS: Immunization with SmpA and PLD both produces a high level of IgG responses to A. baumannii. After immunized with optimal dose of SmpA and PLD, bacterial burden and pulmonary infiltration of inflammatory cells a pneumonia mice model. The immunization with SmpA and PLD also mediates both the cytokine level in BALF and in serum. The antisera from immunization mice can improve the survival curve in mice received lethal infections.

CONCLUSIONS: We identified SmpA and PLD as valuable antigen candidates for the development of an effective vaccine and PLD were also valuable to prepare antisera to control A. baumannii infections.

CLINICAL IMPLICATIONS: Our study may indicated a new way used in the treatment and prophylaxis of A.baumannii infection

DISCLOSURE: The following authors have nothing to disclose: Haitao li, Pinhua Pan

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