Chest Infections: Chest Infections |

Effective Therapy in Immunocompetent Pulmonary Cryptococcosis Patients Restored the Protective Inflammatory Response of the Hosts' Immune Systems FREE TO VIEW

Jinlin Wang, MD; Yunxiang Zeng; Shiyue Li; Xiaohong Xie
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The State Key Laboratory of Respiratory Disease, China Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A85. doi:10.1016/j.chest.2016.02.090
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SESSION TITLE: Chest Infections

SESSION TYPE: Original Investigation Slide

PRESENTED ON: Saturday, April 16, 2016 at 02:15 PM - 03:45 PM

PURPOSE: The dynamics of cytokine production in immunocompetent patients with pulmonary cryptococcosis (PC) were investigated, and the mechanism underlying the reduction of the protective immune response of the patients’ peripheral blood mononuclear cells (PBMCs) was examined.

METHODS: The concentration of the serum interferon-γ (IFN-γ) and interleukin (IL)-2, -4, -10 and -12 was measured at the baseline and after treatment for 6 months. The blood samples were extracted from 30 immunocompetent patients with PC and from 30 age- and gender-matched healthy controls. PBMCs were isolated and incubated with or without recombinant human IL-12 (rhIL-12) for 48 h, and the concentrations of IFN-γ and IL-4 in the supernatant were subsequently measured.

RESULTS: Baseline serum IFN-γ levels were significantly reduced in the PC patients compared with the control group (P = 0.000). The serum IL-2 and IFN-γ levels were significantly higher after thorough treatment than the baseline (P = 0.022 and P = 0.000). The baseline concentrations of IFN-γ and IL-4 in the supernatant of PBMCs without rhIL-12 stimulation were not different between the two groups. rhIL-12 stimulated the release of IFN-γ but not of IL-4; the increase in IFN-γ levels was lower in the patients than in the controls (P = 0.002). After the thorough treatment, rhIL-12 stimulation increased the median levels of the supernatant IFN-γ by 6.08 ± 0.39-fold compared with the baseline (P = 0.003).

CONCLUSIONS: Immunocompetent PC patients exhibited reduced serum IFN-γ levels, although it significantly increased after the thorough treatment, which apparently restored the protective inflammatory response of the host’s immune systems. Improved reactivity and sensitivity to the IL-12 stimulation of Th1 cells is possibly one of underlying mechanisms for the changes in the serum IFN-γ levels.

CLINICAL IMPLICATIONS: The study is primarily descriptive and it does focus on the important cytokine (IFN-γ) which has both pathological insights but also so potential therapeutic implications.

DISCLOSURE: The following authors have nothing to disclose: Jinlin Wang, Yunxiang Zeng, Shiyue Li, Xiaohong Xie

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