Allergy and Airway: Asthma IV |

Metabolomics Study on BALF of Allergic Bronchial Asthma Rabbits Treated by Recuperating Lung Decoction FREE TO VIEW

Qi Shi; Xinqiu Wang; Yue Yan; Xinxin Chen; Youlin Li
Author and Funding Information

The Key Institute of state Administration of Traditional Chinese Medicine (Pneumonopathy, Chronic Cough, and Dyspnea), Beijing Key Laboratory (NO. BZ0321), China-Japan Friendship Hospital, Beijing, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A44. doi:10.1016/j.chest.2016.02.046
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: This study was further to complete the method of Recuperating Lung Decoction intervening in the mechanism of asthma on the basis of the successful foundation of rabbit asthma model and the effective model evaluation guided by TCM theory and applying the method of metabolomics.

METHODS: In this study, we established rabbit model of asthma by injecting and atomizing ovalbumin (OVA). Then we combined the evaluation with metabolites changes in BALF detected by GC-MS for a total analysis in order to find possible metabolic markers of asthma.

RESULTS: Results of the score plots showed that the metabolites in the three BALF samples were clearly separated in the quadrant, it was explained there were significant differences among the groups on metabolites. According to the VIP results, the decreasing order of the VIP values was glycine, galactose, palmitic aci, ribitol, aspartate and stearic acid. The variables glycine and galactose had more contributions to the classification. The results of Hierarchical Clustering showed that the blank control group, model group and Recuperating Lung formula group could be distinguished well. A total of 7 kinds of metabolite were detected from the BALF of rabbits: glycine, aspartate, ribitol, galactose, palmitic acid, myristate and stearic acid. One-way ANOVA analysis showed: the metabolites glycine, aspartate, galactose and myristate were different among the three groups.

CONCLUSIONS: The content of some metabolite changed in the model group and treatment group compared with the blank group. It could be speculated that the changing was affected by modeling and drug treatment. According to the result of the study, the increased content of glycine, aspartate and galactose as well as the decreased content of myristic acid in the BALF were closely related to the inflammatory process in the pathogenesis of bronchial asthma. 4 kinds of metabolite including glycine, aspartate, myristic acid and galactose all changed obviously after treated by Recuperating Lung Decoction. Therefore, the changing of the 4 kinds of metabolite above was closely related to the regulation and control of asthma cytokine network by Recuperating Lung Decoction.

CLINICAL IMPLICATIONS: The study has explored a new mode in the study of Recuperating Lung Decoction intervening in the metabolomics changing of asthma animal model, and has got important theoretical and practical significance in deep discussion to TCM clinical and basic theory on the application of the newest technique and achievement of life science.

DISCLOSURE: The following authors have nothing to disclose: Qi Shi, Xinqiu Wang, Yue Yan, Xinxin Chen, Youlin Li

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