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Allergy and Airway: Asthma IV |

Dysregulation of MicroRNAs in Peripheral Blood Mononuclear Cells From Severe Asthmatic Patients FREE TO VIEW

Xiao-Yan Qu; Xiao-Min Dang; Dong Shang; Wei-Jia Wang; Dan Xu; Yong-Ping Shao; Wu-Yuan Lu; Ying Chang
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Center for Translational Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi’an Jiaotong University, Xi'an, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A42. doi:10.1016/j.chest.2016.02.044
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SESSION TITLE: Asthma IV

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Asthma is a chronic inflammatory disease characterized by a reversible airway limitation. Although some studies have investigated the miRNA profiling in animal asthmatic model, there is few study demonstrated the case in human severe asthmatics. In this research, we sought to figure out the miRNA profiling in normal controls versus severe asthmatics.

METHODS: Venous blood from 15 severe asthmatics and 15 normal controls was collected and the peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient centrifugation. Total RNA was isolated and miRNA profiling was detected by using high-throughput miRNA micro-array (Affymetrix miRNA 4.0). The dysregulated miRNAs (fold change≥2) were validated by q-PCR.

RESULTS: The results of miRNA microarray showed that 9 miRNAs were up-regulated and 4 miRNAs were down-regulated in severe asthmatics compared with controls. The up-regulated miRNAs included miR-191-5p, miR-342-3p, miR-92a-3p, let-7b-5p, miR-103a-3p, miR-320a, miR-320b, miR-320c and miR-32, while miR-4516, miR-4454, miR-4668-5p and miR-3613-3p showed down-regulated expression in asthmatic patients. The dysregulation of above mentioned miRNAs were validated by q-RCR.

CONCLUSIONS: The miRNA profiling of severe asthmatic patients was dysregulated.

CLINICAL IMPLICATIONS: The dysregulated miRNAs may provide the potential therapeutic target for severe asthma.

DISCLOSURE: The following authors have nothing to disclose: Xiao-Yan Qu, Xiao-Min Dang, Dong Shang, Wei-Jia Wang, Dan Xu, Yong-Ping Shao, Wu-Yuan Lu, Ying Chang

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