Allergy and Airway: Asthma IV |

The Pathologic Changes of Small Airway in a Neutrophilic Asthma Mouse Model FREE TO VIEW

Wenjin Sun; Xudong Xiang, MD
Author and Funding Information

The Second Xiangya Hospital Of Central South University, Changsha, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A40. doi:10.1016/j.chest.2016.02.042
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To investigate the pathologic changes of small airway in a neutrophilic asthma mouse model

METHODS: Ovalbumin (OVA) and house dust mites (HDM) were applied for sensitization to establish the neutrophilic asthma mouse model, Airway hyperresponsiveness (AHR) was evaluated at 24 h after the last airway challenge. The subtypes and numbers of inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted. Based on small airways (D<500μm) and larger airways (D>500μm), the pathological changes of lung tissues were observed by HE staining, immunohistochemistry of neutrophils, eosinophils, IL-4 and IL-17.

RESULTS: AHR was more significantly in the asthma mouse model (P<0.01), the total number of BALF and neutrophils increased more significantly in the asthma mouse model (P<0.05); Compared with the larger airways, lung inflammatory infiltration, neutrophil infiltration, IL-17 expression was increased significantly in small airways (P<0.05).

CONCLUSIONS: The neutrophilic asthma mouse model with more neutrophil infiltration and IL-17 expression in small airways.

CLINICAL IMPLICATIONS: The pathologic changes of small airway may increased AHR contribute to a more severe asthma phenotype in neutrophilic asthma mouse model.

DISCLOSURE: The following authors have nothing to disclose: Wenjin Sun, Xudong Xiang

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