SESSION TITLE: Asthma IV
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To investigate the pathologic changes of small airway in a neutrophilic asthma mouse model
METHODS: Ovalbumin (OVA) and house dust mites (HDM) were applied for sensitization to establish the neutrophilic asthma mouse model, Airway hyperresponsiveness (AHR) was evaluated at 24 h after the last airway challenge. The subtypes and numbers of inflammatory cells in bronchoalveolar lavage fluid (BALF) were counted. Based on small airways (D<500μm) and larger airways (D＞500μm), the pathological changes of lung tissues were observed by HE staining, immunohistochemistry of neutrophils, eosinophils, IL-4 and IL-17.