Allergy and Airway: Asthma IV |

Relief of Asthma With Regulatory Cells Associated IL-10 and TGF-β FREE TO VIEW

Jing Liu, PhD
Author and Funding Information

The Second Hospital of Jilin University, Changchun, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A35. doi:10.1016/j.chest.2016.02.037
Text Size: A A A
Published online


SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To observe the effect of regulatory cells associated IL-10 and TGF-β on asthma.

METHODS: BABL/c mice were immunized with OVA and alums to induce asthma model. On the provocation process, rhIL-10, rhTGF-β, and rhIL-10+rhTGF-β were given by nose drops from 21 to 28 days. We observed the safety and condition of asthma onset on 28d after inspiration and then did bronchoavleolar lavage to collect lavage fluid, which were used to test the concentration of IL-4, INF-γ and IgE as in serum. The lung tissues were left for pathological examination with HE staining and FOXP3 expression testing with Western blot and rt-PCR.

RESULTS: Low doses of rhIL-10 and rhTGF-βgiven by nose drops were safe to mice. Compared with asthma mice, the intervention with rhIL-10 and rhIL-10+rhTGF-β could decrease the inflammation of lung tissue, eosinophil number and production of IL-4 and IgE in lavage fluid and serum, contrarily increase the FOXP3 gene expression and production of INF-γ (P <0.05). The similar tendency was also shown in the group of intervention with rhTGF-β alone, but not significantly.

CONCLUSIONS: Regulatory T cells could effect asthma through associated IL-10 and TGF-β.

CLINICAL IMPLICATIONS: Low doses of rhIL-10 and rhTGF-β may be an possible treatment to asthma.

DISCLOSURE: The following authors have nothing to disclose: Jing Liu

No Product/Research Disclosure Information




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543