Allergy and Airway: Asthma III |

The Th2 Cell-Promoting Cytokines IL-33 and TSLP, but Not IL-25, Are Potential Biomarkers for Endotypes of Asthma FREE TO VIEW

Wei Wang, PhD; Yan Li; Zhe Lv; Yun Li; Yan Chen; Matt Edwards; Betty Shamji; Kewu Huang; Chris Corrigan; Sun Ying
Author and Funding Information

Capital Medical University, Beijing, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A34. doi:10.1016/j.chest.2016.02.036
Text Size: A A A
Published online


SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: The cytokines IL-33, thymic stromal lymphopoietin (TSLP) and IL-25 have been implicated in asthma pathogenesis owing to the ability to promote Th2-type cytokine synthesis, but their relative importance in “real-life” human asthma is unclear. In the present study, using bronchial alveolar lavage fluid (BALF) as window we aimed to examine airways concentrations of these mediators, along with those of Th1- and Th2-type cytokines in a large group asthmatics (n=70) and controls (n=30), and in the asthmatics compare their concentrations with those of Th1- and Th2-type cytokines, global IgE synthesis and lung function.

METHODS: Analytes were measured in BALF using Meso Scale Discovery (MSD) system and in house ELISA.

RESULTS: The median BALF concentrations of IL-33, TSLP, IL-4, IL-5, IL-13 and IL-12p70, but not IL-25, IL-2 and IFN-g were significantly greater in the asthmatics compared with the controls (p < 0.05). In the asthmatics, the median concentrations of BALF Th2 cytokines were significantly higher in those patients in the middle and upper tertiles of BALF IL-33 and TSLP concentrations compared with those in the lower tertile (p < 0.05). These tertiles of patients also had greater infiltration of BALF eosinophils and neutrophils (p < 0.05) and significantly higher median serum IgE concentrations and lower lung function (FEV1) compared with those in the lower tertile (p < 0.05).

CONCLUSIONS: The data implicate the Th2-promoting cytokines TSLP and IL-33 in a “high inflammation, high Th2, lower lung function and higher global IgE synthesis” endotype of asthma, highlighting them as potential key molecular targets for individualized asthma therapy.

CLINICAL IMPLICATIONS: These anomalies illustrate some of the challenges arising in studies attempting to define asthma “endotypes”, which might be helpful for personalized treatment in asthma.

DISCLOSURE: The following authors have nothing to disclose: Wei Wang, Yan Li, Zhe Lv, Yun Li, Yan Chen, Matt Edwards, Betty Shamji, Kewu Huang, Chris Corrigan, Sun Ying

No Product/Research Disclosure Information




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543