Allergy and Airway: Asthma III |

Pathological ILC2 in Peripheral Blood Acts as an Indicator for the Assessment of Asthma Control in Clinics FREE TO VIEW

Zhihong Chen, MD; Yi Jia; Renhong Tang, PhD; ChunXue Bai, MD; Lei Zhu, MD
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Zhongshan Hospital, Shanghai, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A30. doi:10.1016/j.chest.2016.02.032
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: A newly-discovered type 2 innate lymphoid cell (ILC2), which acted as an early source of Th2 cytokines such as IL-5/IL-13 has been found involved in the pathogenesis of asthma in mice model. However, the potential roles of ILC2 in human asthma are still largely unknown. In this study, we will investigate the correlation between pathological ILC2s and asthma clinical characteristics. Cytokine profiles of ILC2 in response to stimulators released by epithelial cell will also be determined.

METHODS: A total of 42 persistent asthma and 26 healthy subjects were enrolled in this study. ILC2s from human mononuclear cells in peripheral blood (PBMCs) were identified through flow-cytometric analysis. Frequency of IL-13+ILC2 in the groups of asthmatics and healthy donors were checked by FACS intracellular staining. Sorted ILC2s were stimulated with IL-25 plus IL-33 in different time points, cytokine profiles were checked by multiplex ELISA; Correlation between IL-13+ILC2 and varied data from clinics were analyzed.

RESULTS: Human ILC2s were identified successfully as Lin-CD45highIL-7R+CRTH2+ cells, the percentages of ILC2s were 0.022±0.016% in asthmatics and 0.016±0.007% in healthy donors respectively. Percentages of IL-13+ILC2 were significantly higher in PBMCs of poorly-controlled group (53.1±16.3%) or part-control group (37.9+11.7) than well-controlled group (17.9±6.33%) and healthy controls (18.2±9.4%) group (p<0.05). Sorted ILC2s showed high IL-5- and IL-13-producing capabilities in response to IL-25+IL-33. Pathological ILC2 (IL-13+ILC2) was positively correlated with asthma control systems (GINA 2006 and 2015 criteria) (R2=0.56, 0.62 respectively; p<0.05).

CONCLUSIONS: The frequency of pathological IL-13+ILC2 was positive correlation with the control status of asthma which has great potential to be an objective indicator for assessing asthma control in the future.

CLINICAL IMPLICATIONS: 1. to expand the clinical implication of ILC2. 2. peripheral ILC2 has a great potential to be a biomarker for assessing asthma control in the future.

DISCLOSURE: The following authors have nothing to disclose: Zhihong Chen, Yi Jia, Renhong Tang, ChunXue Bai, Lei Zhu

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