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Allergy and Airway: Asthma III |

Effect of Transient Receptor Potential Vanihoid-1(TRPV1) From Central Nervous System (CNS) on Guinea Pigs With Chronic Cough Induced by PM2.5

Haining Lv; Jianliang Yue; Zhe Chen; Senlin Chai; Xu Cao; Jie Zhan; Hui Zhang; Rong Dong; KeFang Lai
Author and Funding Information

Medical School of Southeast University, Nanjing, China


Copyright 2016, American College of Chest Physicians. All Rights Reserved.


Chest. 2016;149(4_S):A25. doi:10.1016/j.chest.2016.02.027
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SESSION TITLE: Asthma III

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To investigate the effect of TRPV1 from CNS on guinea pigs with chronic cough induced by PM2.5

METHODS: The models of guinea pigs exposed to PM2.5 for 21 days were established and followed by the evaluation of their cough reflex sensitivity via examining the concentrations of citric acid inducing at least 5 coughs (C5). The changes of inflammatory in the airway were observed by HE staining and airway vascular permeability was detected by Evans blue fluorescence. The expressions of substance P (SP) in the airway which is a neuro-transmitter for airway neurogenic inflammation were observed by immunohistochemistry. The expressions of TRPV1 from Botzinger complex and vagal complex in the medulla oblongata were observed by immunofluorescence. TRPV1 agonist, Capsaicine and TRPV1 antagonist, Capsazepine were respectively micro-injected into the vagal complex in the medulla oblongata and the changes of cough reflex sensitivity and airway neurogenic inflammation were detected afterwards.

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